Aging and fasting regulation of leptin and hypothalamic neuropeptide Y gene expression
Aging and fasting regulation of leptin and hypothalamic neuropeptide Y gene expression
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DOI：
10.1152/ajpendo.1998.275.3.e405
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发表时间：
1998-09-01
影响因子：
5.1
通讯作者：
Scarpace, PJ
中科院分区：
文献类型：
--
作者：
Li, H;Matheny, M;Scarpace, PJ
为了研究衰老对禁食诱导的瘦素基因表达抑制和下丘脑神经肽 Y (NPY) 基因表达增加的作用，我们对 3、24 和 31 个月的雄性 F-344xBN 大鼠禁食或随意喂养 2 天。我们检测了腹膜后、腹股沟和附睾白色脂肪组织 (WAT) 中的瘦素 mRNA 水平；血清瘦素水平；和下丘脑中 NPY mRNA 水平。我们发现，腹膜后 WAT 和腹股沟 WAT 中瘦素 mRNA 水平从 3 个月到 24 个月增加，并在 24 个月到 31 个月之间趋于平稳，但附睾 WAT 中瘦素 mRNA 水平随着年龄的增长而变化。血清瘦素水平随年龄增加而增加，而下丘脑 NPY mRNA 水平不随年龄变化。禁食抑制所有三种 WAT 和血清瘦素中的瘦素基因表达。此外，老年大鼠中血清瘦素和腹膜后 WAT 中瘦素信息的抑制较少。相反，禁食会增加下丘脑 NPY 信息，但在老年大鼠中，程度较小。在进食（随意）和禁食的大鼠中，年轻大鼠的血清瘦素和下丘脑 NPY mRNA 水平之间存在很强的相关性，但在两个老年组中均没有这种相关性。这些数据表明老年 F-344xBN 大鼠具有瘦素抵抗性，并且老年大鼠血清瘦素、瘦素 mRNA 和下丘脑 NPY mRNA 的空腹调节受损。
To investigate the role of aging on the fasting-induced suppression of leptin gene expression and increase in hypothalamic neuropeptide Y (NPY) gene expression, we fasted or fed ad libitum male F-344xBN rats aged 3, 24, and 31 mo for 2 days. We examined leptin mRNA levels in retroperitoneal, inguinal, and epididymal white adipose tissue (WAT); serum leptin levels; and NPY mRNA levels in the hypothalamus. We found that leptin mRNA levels were increased from 3 to 24 mo and leveled off between 24 and 31 mo in both retroperitoneal WAT and inguinal WAT but were unchanged with age in epididymal WAT. Serum leptin levels increased with age, whereas hypothalamic NPY mRNA levels did not change with age. Fasting suppressed leptin gene expression in all three WATs and serum leptin. Moreover, this suppression of serum leptin and of leptin message in retroperitoneal WAT was less in aged rats. Conversely, fasting increased hypothalamic NPY message, again to a lesser extent in aged rats. In both fed (ad libitum) and fasted rats, there was a strong correlation between serum leptin and hypothalamic NPY mRNA levels in the young but not in either of the two aged groups. These data suggest that aged F-344xBN rats are leptin resistant and that the fasting regulation of serum leptin, leptin mRNA, and hypothalamic NPY mRNA is impaired in aged rats.