p63 is a prostate basal cell marker and is required for prostate development
p63 is a prostate basal cell marker and is required for prostate development
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DOI：
10.1016/s0002-9440(10)64814-6
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发表时间：
2000-12-01
影响因子：
6
通讯作者：
Loda, M
中科院分区：
文献类型：
--
作者：
Signoretti, S;Waltregny, D;Loda, M
p53的同系物p63编码不同的同型，既可以反激活p53报告基因（TAP63），也可以作为p53的显性阴性基因（Delta Np63）。P63在许多上皮器官的基底细胞中表达，在小鼠中，它的种系失活导致皮肤附属物和乳房等器官发育不全。在这里，我们发现前列腺基底细胞，而不是分泌细胞或神经内分泌细胞，表达p63。此外，培养的前列腺基底细胞主要表达Delta Np63 α同型，相比之下，p63蛋白在人前列腺腺癌中未被检测到。最后，也是最重要的，p63（-/-）小鼠不发育前列腺。这些结果表明p63是前列腺发育所必需的，并支持基底细胞代表和/或包括前列腺干细胞的假设。此外，我们的研究结果表明p63免疫组织化学可能是鉴别前列腺良恶性病变的一种有价值的工具。
The p53 homologue p63 encodes for different isotypes able to either transactivate p53 reporter genes (TAP63) or act as p53-dominant-nebatives (Delta Np63). p63 is expressed in the basal cells of many epithelial organs and its germline inactivation in the mouse results in agenesis of organs such as skin appendages and the breast. Here, we show that prostate basal cells, but not secretory or neuroendocrine cells, express p63, In addition, prostate basal cells in culture predominantly express the Delta Np63 alpha isotype, In contrast, p63 protein is not detected in human prostate adenocarcinomas. Finally, and most importantly, p63(-/-) mice do not develop the prostate. These results indicate that p63 is required for prostate development and support the hypothesis that basal cells represent and/or include prostate stem cells. Furthermore, our results show that p63 immunohistochemistry may be a valuable tool in the differential diagnosis of benign versus malignant prostatic lesions.