Water regulation by a presumptive hormone contained in identified neurosecretory cell R15 of Aplysia.
Water regulation by a presumptive hormone contained in identified neurosecretory cell R15 of Aplysia.
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DOI：
10.1085/jgp.67.1.113
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发表时间：
1976-01
影响因子：
3.8
通讯作者：
Weiss, K
中科院分区：
文献类型：
--
作者：
Kupfermann, I;Weiss, K
注射的匀浆确定的神经元R15的血脑膨出的Aaplasia产生的重量增加3-10%，在90分钟内。其他几个确定的神经元或海水的控制注射，是无效的。即使动物保持在5%高渗海水中，体重也会增加。在酸化至pH 2并加热至100 ℃后，R15匀浆的活性得以保留;但通过链霉蛋白酶的蛋白水解消化，活性被破坏。在纤维素透析管中透析导致Sephadex G-50上的aion的显著损失（标称排除极限1，500 - 30，000道尔顿），活性存在于部分包含的体积中，但在完全排除或完全包含的体积中不存在。这些数据支持R15含有一种或多种参与离子调节或水平衡的激素的观点。R15提取物经过不同处理后的生物测定结果与活性是由于一种或多种相对低分子量的稳定多肽的假设一致。
Injection of an homogenate of identified neuron R15 into the hemocele of Aplysia produced a weight increase of 3-10% within 90 min. Control injections of several other identified neurons or of seawater, were ineffective. The weight increase occurred even when the animals were maintained in 5% hyperosmotic seawater. The activity of the R15 homogenate was retained after acidification to pH 2 and heating to 100 degrees C; but activity was destroyed by proteolytic digestion with Pronase. Dialysis in cellulose dialysis tubing resulted in a significant loss of aion on Sephadex G-50 (nominal exclusion limits 1,500-30,000 daltons), activity was present in the partially included volumes, but was absent in the totally excluded or totally included volumes. The data support the notion that R15 contains one or more hormones involved in ionic regulation or water balance. The results of bioassays of R15 extracts subjected to different treatments are consistent with the hypothesis that activity is due to one or more stable polypeptides of relatively low molecular weight.