Multiple high risk HPV infections are common in cervical neoplasia and young women in a cervical screening population
Multiple high risk HPV infections are common in cervical neoplasia and young women in a cervical screening population
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DOI：
10.1136/jcp.57.1.68
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发表时间：
2004-01-01
影响因子：
3.4
通讯作者：
McGoogan, E
中科院分区：
文献类型：
--
作者：
Cuschieri, KS;Cubie, HA;McGoogan, E
目的：如果将人乳头瘤病毒（HPV）检测纳入宫颈筛查计划，则需要确定多种HPV感染在宫颈肿瘤中的重要性。本研究调查了多种HPV类型的多样性在常规宫颈筛查人群，并评估与宫颈neoplasia.Methods：总体HPV的患病率，类型特异性患病率，和多重感染的程度进行了评估，从3444液体为基础的细胞学样本的残留材料，使用真实的时间GP 5 +/GP 6+聚合酶链反应筛查和线性阵列基因分型。HPV状态进行了研究，年龄和并发细胞学证据dyskaryosis.Results：百分之二十的样本HPV阳性。HPV类型多样性广泛，并且在一半的HPV阳性样本中发生多种HPV感染。年轻女性更容易感染多重高危HPV（HR-HPV）。在3.4%的肿瘤阴性样本和33.3%、41.8%和40.4%的交界性、轻度或高度核异常样本中发现了多种HR-HPV类型的感染。单一HR-HPV感染被发现在4.9%，38.6%，45.0%，和51.1%的阴性，临界，轻度，或高度dyskaryosis samples.Conclusions：多HR-HPV感染是最流行的年轻女性。多重HR-HPV感染在高级别宫颈瘤变中并不比低级别宫颈瘤变中更常见，反映了多重HR-HPV的常见性传播。需要进行前瞻性队列研究，将HPV类型的连续丢失或获得与细胞学分析联系起来，以评估多种HR-HPV感染对肿瘤进展的影响。
Aims: If human papillomavirus (HPV) testing is to be included within cervical screening programmes, the importance of multiple HPV infections in cervical neoplasia needs to be determined. This study investigated the diversity of multiple HPV types in a routine cervical screening population, and assessed associations with cervical neoplasia.Methods: Overall HPV prevalence, type specific prevalence, and extent of multiple infection were assessed in residual material from 3444 liquid based cytology samples, using real time GP5+/GP6+ polymerase chain reaction for screening and linear array assay for genotyping. HPV status was studied in relation to age and concurrent cytological evidence of dyskaryosis.Results: Twenty per cent of samples were HPV positive. HPV type diversity was broad, and multiple HPV infections occurred in half of the HPV positive samples. Younger women were significantly more likely to harbour multiple high risk HPV (HR-HPV) infections. Infections with multiple HR-HPV types were found in 3.4% of samples negative for neoplasia and in 33.3%, 41.8%, and 40.4% of samples with borderline, mild, or high grade dyskaryosis, respectively. Single HR-HPV infections were found in 4.9%, 38.6%, 45.0%, and 51.1% of negative, borderline, mild, or high grade dyskaryosis samples, respectively.Conclusions: Multiple HR-HPV infections were most prevalent in young women. Multiple HR-HPV infections were not more frequent in high grade than in low grade cervical neoplasia, reflecting common sexual transmission of multiple HR-HPV. Prospective cohort studies linking sequential loss or gain of HPV types with cytological analysis are required to assess the impact of multiple HR-HPV infections on neoplastic progression.