Activation of human gamma delta T-cells by heat-treated mistletoe plant extracts
Activation of human gamma delta T-cells by heat-treated mistletoe plant extracts
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DOI：
10.1016/0165-2478(96)02584-9
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发表时间：
1996-09-01
影响因子：
4.4
通讯作者：
Kabelitz, D
中科院分区：
文献类型：
--
作者：
Fischer, S;Scheffler, A;Kabelitz, D
已知包括分枝杆菌、其它细菌和寄生虫（如恶性疟原虫）的各种微生物在体外激活人γ δ T细胞。在这项研究中，我们证明，热处理（但不是未经处理）槲寄生提取物类似地刺激人类γ δ T细胞在体外培养。应答T细胞表达可变T细胞受体元件V γ 9和V δ 2。热处理槲寄生提取物的γ δ刺激活性对碱性磷酸酶处理敏感，但对蛋白酶K不敏感，这表明配体是非蛋白质的含磷酸盐化合物。槲寄生衍生的配体与先前定义的用于γ δ T细胞的分枝杆菌衍生的配体共享这些特征。
Various microorganisms including mycobacteria, other bacteria, and parasites such as Plasmodium falciparum are known to activate human gamma delta T-cells in vitro. In this study, we demonstrate that heat-treated (but not untreated) mistletoe extracts similarly stimulate human gamma delta T-cells during in vitro culture. The responding T-cells express the variable T-cell receptor elements V gamma 9 and V delta 2. The gamma delta-stimulating activity of heat-treated mistletoe extracts is sensitive to treatment with alkaline phosphatase but not with proteinase K, indicating that the ligands are non-proteinaceous phosphate-containing compounds. Mistletoe-derived ligands share these features with the previously defined mycobacteria-derived ligands for gamma delta T-cells.