In vivo imaging of hydrogen peroxide with chemiluminescent nanoparticles
In vivo imaging of hydrogen peroxide with chemiluminescent nanoparticles
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DOI：
10.1038/nmat1983
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发表时间：
2007-10-01
期刊：
影响因子：
41.2
通讯作者：
Murthy, Niren
中科院分区：
文献类型：
--
作者：
Lee, Dongwon;Khaja, Sirajud;Murthy, Niren
过氧化氢的过度产生与许多疾病的发展有关1 -4，目前人们对开发能够在体内对过氧化氢成像的造影剂非常感兴趣。在这份报告中，我们证明了纳米粒子配制过草酸酯和荧光染料可以成像过氧化氢在体内具有高特异性和灵敏度。过草酸盐纳米颗粒通过过氧化氢、过草酸酯和荧光染料之间的三组分化学发光反应对过氧化氢进行成像。过草酸盐纳米颗粒具有用于体内成像的几个有吸引力的性质，例如可调谐波长发射（460-630 nm）、对过氧化氢的纳摩尔灵敏度和对过氧化氢优于其它活性氧物质的优异特异性。过草酸盐纳米颗粒能够在脂多糖诱导的炎症反应期间对小鼠腹腔中的过氧化氢进行成像。我们预计过草酸盐纳米粒子的体内过氧化氢成像的许多应用，因为它们的高特异性和灵敏度和深层组织成像能力。
The overproduction of hydrogen peroxide is implicated in the development of numerous diseases1-4 and there is currently great interest in developing contrast agents that can image hydrogen peroxide in vivo. In this report, we demonstrate that nanoparticles formulated from peroxalate esters and fluorescent dyes can image hydrogen peroxide in vivo with high specificity and sensitivity. The peroxalate nanoparticles image hydrogen peroxide by undergoing a three-component chemiluminescent reaction between hydrogen peroxide, peroxalate esters and fluorescent dyes. The peroxalate nanoparticles have several attractive properties for in vivo imaging, such as tunable wavelength emission (460-630 nm), nanomolar sensitivity for hydrogen peroxide and excellent specificity for hydrogen peroxide over other reactive oxygen species. The peroxalate nanoparticles were capable of imaging hydrogen peroxide in the peritoneal cavity of mice during a lipopolysaccharide-induced inflammatory response. We anticipate numerous applications of peroxalate nanoparticles for in vivo imaging of hydrogen peroxide, given their high specificity and sensitivity and deep-tissue-imaging capability.