Topographical specificity of regulation of respiratory and renal sympathetic activity by the midbrain dorsolateral periaqueductal gray
Topographical specificity of regulation of respiratory and renal sympathetic activity by the midbrain dorsolateral periaqueductal gray
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DOI：
10.1152/ajpregu.00249.2010
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发表时间：
2010-09-01
影响因子：
2.8
通讯作者：
Dampney, Roger A. L.
中科院分区：
文献类型：
--
作者：
Iigaya, Kamon;Horiuchi, Jouji;Dampney, Roger A. L.
刘建军，李建军，李建军，等。中脑导水管周围背外侧灰质调节呼吸和肾交感神经活动的地形特异性。[J] .中国生物医学工程学报，2016,33(2):559 - 561。首次发表于2010年5月26日；doi: 10.1152 / ajpregu.00249.2010。中脑导水管周围灰质（PAG）介导对各种应激源的生理反应。它由四个纵向柱组成，具有不同的解剖连接和功能特性。先前的解剖学和行为学研究已经提出了这样的假设，即背外侧PAG，而不是相邻的外侧和背内侧亚区，是整合急性心理威胁刺激行为反应的关键中心。在这项研究中，我们测试了背外侧PAG神经元的激活是否与周围区域的心血管和呼吸反应模式不同，是否与这一假设一致。同时记录麻醉大鼠动脉压、心率、肾交感神经活动（RSNA）和膈神经活动（PNA）。微量注射D， l -同型半胱酸（750 pmol / 15 nl）在整个背内侧、背外侧和外侧PAG亚区。所有三个PAG亚区均诱发了类似幅度的RSNA升高，并伴有小到中度的动脉压和心率升高。相比之下，PNA爆发率（呼吸率）和整体呼吸活动的大幅增加仅在高度限定的区域引起，该区域在中尾侧水平上与背外侧PAG亚区密切相关。在这一区域，RSNA的升高与呼吸活动高度相关（r = 0.914, P < 0.001），这表明PAG背外侧的普通“命令神经元”群可能从这一区域产生交感和呼吸反应。
Iigaya K, Horiuchi J, McDowall LM, Dampney RAL. Topographical specificity of regulation of respiratory and renal sympathetic activity by the midbrain dorsolateral periaqueductal gray. Am J Physiol Regul Integr Comp Physiol 299: R853-R861, 2010. First published May 26, 2010; doi:10.1152/ajpregu.00249.2010.-The midbrain periaqueductal gray (PAG) mediates the physiological responses to a wide range of stressors. It consists of four longitudinal columns that have different anatomical connections and functional properties. Previous anatomical and behavioral studies have led to the hypothesis that the dorsolateral PAG, but not the adjacent lateral and dorsomedial subregions, is a key center that integrates the behavioral response to acute psychological threatening stimuli. In this study, we tested whether, consistent with this hypothesis, activation of neurons in the dorsolateral PAG evokes a pattern of cardiovascular and respiratory responses that is distinct from that evoked from surrounding regions. Arterial pressure, heart rate, renal sympathetic nerve activity (RSNA), and phrenic nerve activity (PNA) were recorded simultaneously in urethane-anesthetized rats. Microinjections of very small amounts of D, L-homocysteic acid (750 pmol in 15 nl) were made in sites throughout the dorsomedial, dorsolateral, and lateral PAG subregions. Increases in RSNA of similar magnitude accompanied by small to moderate increases in arterial pressure and heart rate were evoked from all three PAG subregions. In contrast, large increases in both PNA burst rate (respiratory rate) and overall respiratory activity were evoked only from a highly circumscribed region that corresponded closely to the dorsolateral PAG subregion at an intermediate to caudal level. Within this region, the evoked increases in RSNA and respiratory activity were highly correlated (r = 0.914, P < 0.001), suggesting the possibility that a common population of "command neurons" within the dorsolateral PAG may generate both sympathetic and respiratory responses from this region.