ALPHA-AMANITIN-RESISTANT VIRAL-RNA SYNTHESIS IN NUCLEI ISOLATED FROM NUCLEAR POLYHEDROSIS VIRUS-INFECTED HELIOTHIS-ZEA LARVAE AND SPODOPTERA-FRUGIPERDA CELLS
ALPHA-AMANITIN-RESISTANT VIRAL-RNA SYNTHESIS IN NUCLEI ISOLATED FROM NUCLEAR POLYHEDROSIS VIRUS-INFECTED HELIOTHIS-ZEA LARVAE AND SPODOPTERA-FRUGIPERDA CELLS
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DOI：
10.1128/jvi.38.3.916-921.1981
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
WEAVER, RF
中科院分区：
文献类型：
--
作者：
GRULA, MA;BULLER, PL;WEAVER, RF
[3 H]RNA在感染后不同时间从H.感染H.玉米核型多角体病毒（NPV）和培养的S.用苜蓿银纹夜蛾核型多角体病毒（Autographa californica NPV）感染草地贪夜蛾细胞。为了检测病毒特异性RNA合成，将[3 H]RNA与固定在硝酸纤维素滤膜上的变性病毒DNA杂交。从H. zea幼虫脂肪体和S.浓度的α-葡聚糖抑制了20-25%的草地贪夜蛾细胞。鹅膏蕈碱足以抑制宿主RNA聚合酶II。在S.在存在α-鹅膏蕈碱浓度抑制90%的细胞RNA聚合酶II活性。细胞RNA聚合酶II酶保持对α-在感染期间存在鹅膏蕈碱，并且没有证据表明病毒编码的α-鹅膏蕈碱鹅膏蕈碱抗性酶在感染开始后合成。显然，在分离的细胞核中，大部分的NPV特异性RNA合成是由宿主RNA聚合酶II以外的酶转录的。
[3H]RNA was synthesized in nuclei isolated at various times postinfection from the fat bodies of H. zea larvae infected with H. zea nuclear polyhedrosis virus (NPV) and from cultured S. frugiperda cells infected with Autographa californica NPV. To detect virus-specific RNA synthesis, the [3H]RNA was hybridized to denatured viral DNA immobilized on nitrocellulose filters. NPV-specific RNA synthesis in the infected nuclei isolated from H. zea larval fat bodies and S. frugiperda cells was inhibited 20-25% by concentrations of .alpha.-amanitin sufficient to inhibit the host RNA polymerase II. A productive NPV infection was obtained in S. frugiperda cells grown in the presence of an .alpha.-amanitin concentration that inhibited 90% of the cellular RNA polymerase II activity. The cellular RNA polymerase II enzyme remained sensitive to .alpha.-amanitin during infection and there was no evidence that a virus-coded, .alpha.-amanitin-resistant enzyme was synthesized after the onset of infection. Evidently the bulk of NPV-specfic RNA synthesis in isolated nuclei is transcribed by an enzyme other than the host RNA polymerase II.