The claustrum coordinates cortical slow-wave activity
The claustrum coordinates cortical slow-wave activity
复制标题
DOI：
10.1038/s41593-020-0625-7
复制
发表时间：
2020-05-11
影响因子：
25
通讯作者：
Yoshihara, Yoshihiro
中科院分区：
文献类型：
--
作者：
Narikiyo, Kimiya;Mizuguchi, Rumiko;Yoshihara, Yoshihiro
在睡眠和清醒休息期间，新皮层产生大规模的慢波（SW）活动。在这里，我们报告说，屏状核协调新皮层SW的产生。我们建立了一个转基因小鼠系，使基因审问的一个亚群的幽闭mamatergic神经元。这些神经元从广泛的新皮层区域接收输入，并向其发送输出。幽闭层神经元的放电主要与皮层SW活动相关。在体外光遗传学刺激屏状核诱导兴奋性突触后反应，在大多数新皮层神经元，但引起的动作电位主要在抑制性中间神经元。体内光遗传学刺激诱导了一个同步的向下状态，其特征在于许多皮层区域的所有层中的神经活动的长时间沉默，然后是向下到向上的状态转换。相比之下，基因消融的锁状神经元衰减SW活动在额叶皮层。这些结果表明，在广泛的皮层区域同步抑制性中间神经元的SW activity. The时空协调的claustrum-Cre转基因小鼠线，并证明，屏状神经元的关键作用，通过同步驱动广泛的皮层区域的抑制性中间神经元，屏状管弦乐皮层慢波活动。
During sleep and awake rest, the neocortex generates large-scale slow-wave (SW) activity. Here, we report that the claustrum coordinates neocortical SW generation. We established a transgenic mouse line that enabled the genetic interrogation of a subpopulation of claustral glutamatergic neurons. These neurons received inputs from and sent outputs to widespread neocortical areas. The claustral neuronal firings mostly correlated with cortical SW activity. In vitro optogenetic stimulation of the claustrum induced excitatory postsynaptic responses in most neocortical neurons, but elicited action potentials primarily in inhibitory interneurons. In vivo optogenetic stimulation induced a synchronized down-state featuring prolonged silencing of neural activity in all layers of many cortical areas, followed by a down-to-up state transition. In contrast, genetic ablation of claustral neurons attenuated SW activity in the frontal cortex. These results demonstrate a crucial role of claustral neurons in synchronizing inhibitory interneurons across wide cortical areas for the spatiotemporal coordination of SW activity.The authors establish the claustrum-Cre transgenic mouse line and demonstrate that the claustrum orchestrates cortical slow-wave activity by synchronously driving the inhibitory interneurons in widespread cortical areas.