Significance of M2 macrophages in glomerulonephritis with crescents
Significance of M2 macrophages in glomerulonephritis with crescents
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DOI：
10.1016/j.prp.2017.04.011
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发表时间：
2017-01-01
影响因子：
2.8
通讯作者：
Wang, Cui-Mei
中科院分区：
文献类型：
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作者：
Li, Jun;Yu, Ya-Fen;Wang, Cui-Mei
目的：CD 163和CD 206是M2巨噬细胞的标志物，具有抗炎作用。本研究旨在探讨M2巨噬细胞在新月体肾炎患者中的临床病理意义。来自肾小球肾炎患者的肾组织样本，超过30%的细胞或细胞纤维新月体，包括狼疮肾炎（LN，n = 14），抗中性粒细胞胞浆抗体相关性血管炎本研究包括腺相关病毒（AAV）14例，伊加肾病（IgAN）11例，过敏性紫癜性肾炎（HSPGN）8例。结果：（1）CD 163主要表达于细胞性或细胞纤维性新月体、增生性肾小球病变和急性肾小管间质损伤; LN和AAV中CD 163阳性细胞数多于IgAN和HSPGN。(2)CD 206阳性细胞主要见于急性肾小管间质损伤和增生性肾小球病变，尤其是LN。LN患者肾小球内CD 206阳性细胞数明显多于其他各组。LN和AAV急性肾小管间质病变中CD 163和CD 206阳性细胞数均高于IgAN和HSPGN。(3)急性肾小管间质病变中CD 163阳性细胞和CD 206阳性细胞的数量与估计的肾小球滤过率呈负相关。(4)在LN中，活动指数（AI）与CD 206阳性细胞和CD 163阳性细胞的数量呈正相关。CD 163阳性细胞和CD 206阳性细胞同时表达CD 68。结论：CD 163阳性细胞和CD 206阳性细胞是巨噬细胞亚群，参与活动性新月体肾炎的发病过程，尤其是在LN和AAV中。(C)2017 Elsevier GmbH. All rights reserved.
Objectives: CD163 and CD206, markers of M2 macrophages, possesses anti-inflammatory properties. This study aims to investigate the clinicopathologic significance of M2 macrophages in patients of glomerulonephritis with crescents.Methods: Renal tissue samples from patients of glomerulonephritis with more than 30% cell or cell fibrous crescents, including lupus nephritis (LN, n = 14), anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV, n = 14), IgA nephropathy(IgAN) (n = 11), Henoch Schonlein purpura glomerulonephritis(HSPGN)(n = 8)were included in this study. The expression of CD163, CD206 and CD68 in renal tissues was detected by immunohistochemistry or immunofluorescence.Results: (1) CD163 was mainly expressed in cell or cell-fibrous crescents, proliferative glomerular lesions and acute tubulointerstitial injury. There were numerous CD163-positive cells in LN and AAV in comparison with IgAN and HSPGN. (2) CD206-positive cells were mainly observed in acute tubulointerstitial injury, and proliferative glomerular lesions, especially in LN. Patients with LN had numerous CD206-positive cells in glomerular than other groups. The number of CD163-positive cells and CD206-positive cells in acute tubulointerstitial lesions of LN and AAV were more than IgAN and HSPGN. (3) Both the number of CD163-positive cells and CD206-positive cells in acute tubulointerstitial lesions negatively correlated to estimated glomerular filtration rate. (4) In LN, activity index (AI) positively correlated with the number of CD206-positive cells and CD163-positive cells. Dual staining showed that CD163-positive cells and CD206-positive cells also expressed CD68.Conclusions: CD163-positive cells and CD206-positive cells, subpopulation of macrophages, which were involved in the pathogenesis of active crescentic glomerulonephritis, especially in LN and AAV. (C) 2017 Elsevier GmbH. All rights reserved.