Comprehensive Guide to Extracting and Expressing Fungal Secondary Metabolites with Aspergillus fumigatus as a Case Study.
Comprehensive Guide to Extracting and Expressing Fungal Secondary Metabolites with Aspergillus fumigatus as a Case Study.
复制标题
DOI：
10.1002/cpz1.321
复制
发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Keller NP
中科院分区：
文献类型：
--
作者：
Nickles G;Ludwikoski I;Bok JW;Keller NP
真菌次生代谢产物（SM）已经引起了学术界和工业界天然产物研究人员的兴趣几十年。近年来，先前表征的代谢物的高重新发现率使得发现新化合物变得越来越困难。此外，绝大多数真菌SM存在于遗传上难处理的真菌中，或者在遗传上易处理的真菌中在正常实验室条件下沉默。真菌天然产物社区通过改变真菌的物理生长条件和生物合成基因簇调节剂或蛋白质的异源/同源表达，广泛地克服了这些障碍。这里描述的协议总结了研究真菌中次级代谢产物生产时所需的重要方法。我们还总结了烟曲霉中每种已发表的次级代谢产物的生长条件、遗传背景和提取方案，使读者能够轻松复制先前表征的SM的生产。读者还将配备用于开发他们自己的策略的工具，用于从他们给定的真菌或合适的异源模型系统中表达和提取SM。
Fungal secondary metabolites (SM) have captured the interest of natural product researchers in academia and industry for decades. In recent years, the high re-discovery rate of previously characterized metabolites is making it increasingly difficult to uncover novel compounds. Additionally, a vast majority of fungal SMs reside in genetically intractable fungi or are silent in normal laboratory conditions in genetically tractable fungi. The fungal natural products community has broadly overcome these barriers by altering the physical growth conditions of the fungus and heterologous/homologous expression of biosynthetic gene cluster regulators or proteins. The protocols described here summarize vital methodologies needed when researching secondary metabolite production in fungi. We have also summarized the growth conditions, genetic backgrounds, and extraction protocols for every published secondary metabolite in Aspergillus fumigatus, enabling readers to easily replicate the production of previously characterized SMs. Readers will also be equipped with the tools for developing their own strategy for expressing and extracting SMs from their given fungus or suitable heterologous model system.