Calcium regulation of the actin-mediated cytoskeletal transformation of sea urchin coelomocytes.
Calcium regulation of the actin-mediated cytoskeletal transformation of sea urchin coelomocytes.
复制标题
海胆体腔细胞肌动蛋白介导的细胞骨架转化的钙调节。
DOI：
10.1002/cm.970030519
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Schatten,G
中科院分区：
文献类型：
--
作者：
Henson,JH;Schatten,G
几种棘皮动物的体腔细胞一旦受到低渗休克，就会发生肌动蛋白介导的细胞骨架转化。在这项研究中，来自海胆Lytechinus variegatus和Arbacia punctulata的体腔细胞通过在存在外部Ca++的情况下用> 5 μM的钙离子载体A23187处理来诱导“转化”。依赖于外部Ca++和缺乏金霉素染色的离子载体转化表明，这些细胞依赖于外部Ca++源。溶血素透化细胞的NBD-鬼笔毒肽（7-硝基苯-2-氧杂-1，3-二唑-鬼笔毒肽）染色和全细胞透射电子显微镜（TEM）显示，在低渗休克和离子载体转化过程中，肌动蛋白细胞骨架发生了类似的重组，尽管肌动蛋白丝成束在A23187处理的细胞中不太明显。如低渗休克转化所示，离子载体引起的形状变化被抗钙调素药物抑制。大于10 μM浓度的W 13可抑制丝状足的形成，而该药物活性较低的结构类似物W 12则无作用。W 13似乎也破坏了细胞中肌动蛋白丝-膜的结合。荧光定位的钙调素使用的光氧化衍生物的三氟拉嗪表明一般的细胞质分布与一些浓度在丝状核心束。体腔细胞转化可能是Ca++通过钙调蛋白调节肌动蛋白-膜相互作用调节细胞形状变化的一个例子。
Coelomocytes from several echinoderm species undergo an actin‐mediated cytoskeletal transformation once subjected to hypotonic shock. In this study, coelomocytes from the sea urchins Lytechinus variegatus and Arbacia punctulata were induced to “transform” by treatment with > 5 μM of the calcium ionophore A23187 in the presence of external Ca++. The dependence of ionophore transformation on external Ca++and the lack of chlorotetracycline staining indicates that these cells rely on external Ca++sources. NBD‐phallacidin (7‐Nitrobenz‐2‐oxa‐1,3‐diazole‐phallacidin) staining of lysolecithin permeabilized cells and wholemount transmission electron microscopy (TEM) show that similar reorganizations of the actin cytoskeleton take place during hypotonic shock and ionophore transformation, although actin filament bundling is less apparent in A23187‐treated cells. As has been shown with hypotonic shock transformation, the ionophore elicited shape change is inhibited by anticalmodulin drugs. Greater than 10 μM concentrations of W 13 inhibit filopod formation, while this drug's less active structural analogue, W 12, exhibits no effects. W 13 also appears to disrupt actin filament‐membrane associations in the cells. Fluorescent localization of calmodulin using a photooxidized derivative of trifluoperazine indicates a general cytoplasmic distribution with some concentration in filopod core bundles. Coelomocyte transformation may be an example of a cellular shape change regulated by Ca++through the action of calmodulin modulation of actin‐membrane interactions.