Isolation of a cDNA encoding the B isozyme of human phosphoglycerate mutase (PGAM) and characterization of the PGAM gene family.
Isolation of a cDNA encoding the B isozyme of human phosphoglycerate mutase (PGAM) and characterization of the PGAM gene family.
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DOI：
10.1016/s0021-9258(18)37476-3
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发表时间：
1988-11
期刊：
影响因子：
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通讯作者：
S. Sakoda;S. Shanske;S. DiMauro;E. Schon
中科院分区：
文献类型：
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作者：
S. Sakoda;S. Shanske;S. DiMauro;E. Schon
我们以前报道的分离全长cDNA指定肌肉特异性同工酶的人磷酸甘油酸酯（PGAM-M）。我们现在报告的全长cDNA的分离指定的非肌肉特异性，或脑（B），同工酶的人PGAM（PGAM-B）。PGAM-B cDNA编码254个氨基酸，与PGAM-M的同源性为79%，含有913个核苷酸的3′非翻译区，而PGAM-M的3′非翻译区只有37个核苷酸。北方分析证明了PGAM-B转录的非肌肉特异性性质，而基因组Southern分析表明在人类基因组中存在大的PGAM家族。人类和小鼠基因组中的大多数PGAM杂交序列似乎与B-同工酶基因相关;人类PGAM-B基因家族的许多成员显然是加工基因。这些结果与进化分析一致，表明PGAM-B基因是PGAM-M基因的祖先。
We previously reported the isolation of a full-length cDNA specifying the muscle-specific isozyme of human phosphoglycerate mutase (PGAM-M). We now report the isolation of a full-length cDNA specifying the non-muscle-specific, or brain (B), isozyme of human PGAM (PGAM-B). The PGAM-B cDNA encodes a deduced protein 254 amino acids long, 79% identical to PGAM-M, and contains a 913-nucleotide 3′-untranslated region, as compared to the unusually short 37-nucleotide 3′-untranslated region of PGAM-M. Northern analysis demonstrates the non-muscle-specific nature of PGAM-B transcription, while genomic Southern analysis implies the presence of a large PGAM family in the human genome. Most of the PGAM-hybridizing sequences in both the human and mouse genomes seem to be related to the B-isozyme gene; many members of the PGAM-B gene family in humans are apparently processed genes. These results agree with the evolutionary analysis, which indicates that the PGAM-B gene is the progenitor of the PGAM-M gene.