Muscle organization in Caenorhabditis elegans: localization of proteins implicated in thin filament attachment and I-band organization.
Muscle organization in Caenorhabditis elegans: localization of proteins implicated in thin filament attachment and I-band organization.
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DOI：
10.1083/jcb.101.4.1532
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发表时间：
1985-10
期刊：
影响因子：
--
通讯作者：
Waterston RH
中科院分区：
文献类型：
--
作者：
Francis GR;Waterston RH
秀丽隐杆线虫的体壁肌细胞含有斜条纹肌原纤维晶格，其通过两种结构与细胞膜相关联：A带中的M线类似物和I带中的Z盘类似物或致密体。通过使用富含这些结构的级分作为杂交瘤生产的免疫原，我们制备了单克隆抗体，其识别通过免疫荧光和Western转移分析确定的I带的四个组分。致密体的主要成分是与脊椎动物α-辅肌动蛋白共享决定簇的107，000-D多肽。第二种致密体成分是一种更碱性和抗原性不同的107，000-D多肽，其定位于质膜上或质膜下致密体的狭窄结构域。这种基本的致密体多肽也存在于某些细胞边界，其中半带中的细丝终止于称为附着斑的膜相关结构。第三，身份不明的抗原也被发现密切贴附到细胞膜的区域不仅致密体和附着斑块，而且M-线类似物。最后，第四个高分子量抗原，由两个约400，000-D的多肽组成，定位于致密体周围的I带区域。致密体与细胞表面的附着和致密体相关抗原的差异定位表明了它们的组织模型，其中未鉴定的抗原是细胞表面组分，并且两个107，000-D多肽定义了致密体的不同胞质结构域。
The body wall muscle cells of Caenorhabditis elegans contain an obliquely striated myofibrillar lattice that is associated with the cell membrane through two structures: an M-line analogue in the A-band and a Z-disc analogue, or dense-body, in the I-band. By using a fraction enriched in these structures as an immunogen for hybridoma production, we prepared monoclonal antibodies that identify four components of the I-band as determined by immunofluorescence and Western transfer analysis. A major constituent of the dense-body is a 107,000-D polypeptide that shares determinants with vertebrate alpha- actinin. A second dense-body constituent is a more basic and antigenically distinct 107,000-D polypeptide that is localized to a narrow domain of the dense-body at or subjacent to the plasma membrane. This basic dense-body polypeptide is also found at certain cell boundaries where thin filaments in half-bands terminate at membrane- associated structures termed attachment plaques. A third, unidentified antigen is also found closely apposed to the cell membrane in regions of not only the dense-body and attachment plaque, but also the M-line analogue. Finally, a fourth high molecular weight antigen, composed of two polypeptides of approximately 400,000-D, is localized to the I-band regions surrounding the dense-body. The attachment of the dense-body to the cell surface and the differential localization of the dense-body- associated antigens suggest a model for their organization in which the unidentified antigen is a cell surface component, and the two 107,000-D polypeptides define different cytoplasmic domains of the dense-body.