PSEUDOMONAS STIMULATES INTERLEUKIN-8 MESSENGER-RNA EXPRESSION SELECTIVELY IN AIRWAY EPITHELIUM, IN GLAND DUCTS, AND IN RECRUITED NEUTROPHILS
PSEUDOMONAS STIMULATES INTERLEUKIN-8 MESSENGER-RNA EXPRESSION SELECTIVELY IN AIRWAY EPITHELIUM, IN GLAND DUCTS, AND IN RECRUITED NEUTROPHILS
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DOI：
10.1165/ajrcmb.11.6.7946394
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发表时间：
1994-12-01
影响因子：
6.4
通讯作者：
NADEL, JA
中科院分区：
文献类型：
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作者：
INOUE, H;MASSION, PP;NADEL, JA
中性粒细胞可能在慢性气道疾病中起重要作用。假单胞菌是慢性呼吸道疾病的常见病原菌，其在体外可诱导多种细胞表达中性粒细胞趋化因子白细胞介素-8（IL-8）。在这里，我们研究IL-8 mRNA表达的本地化后，孵育人和狗支气管假单胞菌上清液在体外。为了检测IL-8在募集的中性粒细胞中的表达，我们还在体内用假单胞菌上清液灌注狗旁路气管段，并测量腔液中的中性粒细胞数量和IL-8浓度;同时，我们通过导管将假单胞菌上清液引入外周气道。6小时后，我们分析了IL-8 mRNA的表达和定位在切除的组织。未孵育的支气管没有IL-8 mRNA的表达，但在体外假单胞菌培养上清液导致IL-8 mRNA的表达在表面上皮，腺管，和浆液腺细胞的亚群。在体内，引入假单胞菌上清液进入犬气管和外周气道引起上皮和腺管细胞中IL-8 mRNA的表达，但也在招募的中性粒细胞。单用假单胞菌脂多糖在体外和体内均无作用。我们得出结论，假单胞菌的产品，但不是脂多糖，刺激IL-8在气道中的表达，这种表达主要发生在表面上皮细胞和腺管细胞，从而使化学引诱物的细菌网站。此外，募集的中性粒细胞中的IL-8表达为这种保护性抗菌反应的正反馈提供了潜在机制。
Neutrophils may play important roles in chronic airway diseases. Pseudomonas is a common pathogen in some chronic airway diseases, and expression of the neutrophil chemoattractant interleukin-8 (IL-8) is induced by Pseudomonas in various cells in vitro. Here we examine the localization of IL-8 mRNA expression after incubating human and dog bronchi with Pseudomonas supernatant in vitro. To examine IL-8 expression in recruited neutrophils, we also superfused the dog bypassed tracheal segment with Pseudomonas supernatant in vivo and measured neutrophil number and IL-8 concentration in luminal fluid; simultaneously, we introduced Pseudomonas supernatant by catheter in a peripheral airway. After 6 h, we analyzed IL-8 mRNA expression and localization in removed tissue. Unincubated bronchi showed no IL-8 mRNA expression, but incubation with Pseudomonas supernatant in vitro resulted in IL-8 mRNA expression in surface epithelial, gland duct, and a subpopulation of serous gland cells. In vivo, introduction of Pseudomonas supernatant into dog trachea and peripheral airways caused IL-8 mRNA expression in epithelial and gland duct cells but also in the recruited neutrophils. Pseudomonas lipopolysaccharide alone was without effect in vitro and in vivo. We conclude that Pseudomonas products, but not lipopolysaccharide, stimulate IL-8 expression in airways and that this expression occurs primarily in surface epithelial and gland duct cells, thus bringing the chemoattractant to the bacterial site. Furthermore, IL-8 expression in recruited neutrophils provides a potential mechanism for positive feedback of this protective antibacterial response.