Characterization of a Newly Discovered Symbiont of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)
Characterization of a Newly Discovered Symbiont of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)
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新发现的烟粉虱共生体的特征（半翅目：粉虱科）
DOI：
10.1128/aem.03030-12
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发表时间：
2013-01-01
影响因子：
4.4
通讯作者：
Liu, Shu-Sheng
中科院分区：
文献类型：
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作者：
Bing, Xiao-Li;Yang, Jiao;Liu, Shu-Sheng
烟粉虱（Bemisiatabaci）是一个由28种以上隐种组成的物种复合体，其中一些是世界性的重要农作物害虫。与许多其他的吸汁昆虫一样，粉虱也有一个专性共生体，即粉虱和一些次级共生体。到目前为止，在B中已鉴定出6个属的次生共生体。烟粉虱在这项研究中，我们报告和描述的土著B中的一个额外的细菌的发现。烟粉虱隐种中国1号（原名为B.烟粉虱生物型ZHJ 3）。基于16 S rRNA和gltA基因的系统发育分析表明，该细菌属于变形菌门的Alphaproteobacteria亚门，与东方属的人类病原体有密切的关系。因此，我们暂时将其命名为类东方生物（OLO）。OLO在B的8个野生种群中的6个中被发现。烟粉虱中国1号（tabaciChina 1），侵染率为46.2%~ 76.8%。B的荧光原位杂交（FISH）。烟粉虱中国1号（tabaciChina 1）成虫和成虫的OLOs分析表明，OLOs仅存在于细菌组中，与“Ca.花皮藤“通过FISH在卵母细胞的前极端检测到OLO，证明了OLO的垂直传播。种群动态的定量PCR分析表明，“Ca. Portiera aleyrodidarum”和OLO。根据这些结果，我们建议将该共生体从B分类为“亚洲半翅蝗”。烟粉虱
Bemisia tabaci (Hemiptera: Aleyrodidae) is a species complex containing >28 cryptic species, some of which are important crop pests worldwide. Like many other sap-sucking insects, whiteflies harbor an obligatory symbiont, "Candidatus Portiera aleyrodidarum," and a number of secondary symbionts. So far, six genera of secondary symbionts have been identified in B. tabaci. In this study, we report and describe the finding of an additional bacterium in the indigenous B. tabaci cryptic species China 1 (formerly known as B. tabaci biotype ZHJ3). Phylogenetic analysis based on the 16S rRNA and gltA genes showed that the bacterium belongs to the Alphaproteobacteria subdivision of the Proteobacteria and has a close relationship with human pathogens of the genus Orientia. Consequently, we temporarily named it Orientia-like organism (OLO). OLO was found in six of eight wild populations of B. tabaci China 1, with the infection rate ranging from 46.2% to 76.8%. Fluorescence in situ hybridization (FISH) of B. tabaci China 1 in nymphs and adults revealed that OLOs are confined to the bacteriome and co-occur with "Ca. Portiera aleyrodidarum." The vertical transmission of OLO was demonstrated by detection of OLO at the anterior pole end of the oocytes through FISH. Quantitative PCR analysis of population dynamics suggested a complex interaction between "Ca. Portiera aleyrodidarum" and OLO. Based on these results, we propose "Candidatus Hemipteriphilus asiaticus" for the classification of this symbiont from B. tabaci.