Bovine Cumulus Cells Protect Maturing Oocytes from Increased Fatty Acid Levels by Massive Intracellular Lipid Storage
Bovine Cumulus Cells Protect Maturing Oocytes from Increased Fatty Acid Levels by Massive Intracellular Lipid Storage
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DOI：
10.1095/biolreprod.112.106062
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发表时间：
2013-06-01
影响因子：
3.6
通讯作者：
Gadella, Bart M.
中科院分区：
文献类型：
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作者：
Aardema, Hilde;Lolicato, Francesca;Gadella, Bart M.
以血液和卵泡液中游离脂肪酸浓度升高为特征的代谢状况往往与女性生育能力受损有关。特别是，饱和脂肪酸水平升高对几种体细胞类型可能是脂毒性的。本研究的目的是确定卵泡液中游离脂肪酸浓度升高对卵丘细胞和卵母细胞内中性脂(储存在脂滴中的脂肪酸)及其发育能力的影响。为此，奶牛在最终卵母细胞成熟期间接受了一段短期的禁食期。这导致血液和卵泡液中的游离脂肪酸浓度升高，但明显不同，特别是链长度为14-18个碳原子的脂肪酸浓度上升。有趣的是，卵泡液中游离脂肪酸浓度的升高导致卵丘细胞中性脂水平的大量增加，而卵母细胞的中性脂水平几乎没有受到影响。此外，受精和卵丘-卵母细胞复合体培养后卵母细胞发育至囊胚期的能力与对照组无差异。总而言之，这些数据表明，卵泡液中游离脂肪酸浓度的短期升高不会损害卵母细胞的发育能力。我们认为，卵泡液中高水平的油酸结合卵丘细胞中诱导的脂肪储存有助于防止有害的饱和脂肪酸暴露于卵母细胞。
Metabolic conditions characterized by elevated free fatty acid concentrations in blood and follicular fluid are often associated with impaired female fertility. Especially elevated saturated fatty acid levels can be lipotoxic for several somatic cell types. The aim of this study was to determine the impact of elevated free fatty acid concentrations in follicular fluid on neutral lipids (fatty acids stored in lipid droplets) inside cumulus cells and oocytes and their developmental competence. To this end, cows were exposed to a short-term fasting period during final oocyte maturation. This resulted in elevated, but distinct, free fatty acid concentrations in blood and follicular fluid and a rise in the concentrations of in particular fatty acids with a chain length of 14-18 carbon atoms. Interestingly, elevated free fatty acid concentrations in follicular fluid resulted in a massive increase in the level of neutral lipids in cumulus cells, whereas the level of neutral lipid in oocytes was hardly affected. Furthermore, competence of oocytes to develop to the blastocyst stage after fertilization and culture of cumulus-oocyte-complexes of the experimental and control group was not different. In conclusion these data suggest that short-term elevated free fatty acid concentrations in follicular fluid do not harm oocyte developmental competence. We propose that the involvement of high levels of mobilized oleic acid in follicular fluid in combination with the induced lipid storage in cumulus cells serves to prevent harmful saturated fatty acid exposure to the oocyte.