Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue
Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue
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DOI：
10.1002/adbi.202300186
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发表时间：
2023-08-21
期刊：
影响因子：
3.7
通讯作者：
Hinton Jr, Antentor
中科院分区：
文献类型：
--
作者：
Crabtree, Amber;Neikirk, Kit;Hinton Jr, Antentor
线粒体是能量产生所必需的，甚至由于其高铁含量和丰富度而赋予棕色脂肪组织（BAT）其特征颜色。线粒体的生理功能和生物能量能力与其内膜嵴的结构、折叠和组织有关。在衰老过程中，观察到线粒体功能障碍，并且线粒体动力学的调节平衡经常被破坏，导致衰老细胞中线粒体碎片化增加。因此，据推测，BAT线粒体和嵴的显着形态学变化将与衰老。一个定量的三维电子显微镜的方法来映射嵴网络组织在小鼠BAT测试这一假设。使用这种方法，线粒体嵴的3D形态研究在成年（3个月）和老年（2岁）鼠BAT组织通过连续块面扫描电子显微镜（SBF-SEM）和3D重建软件手动分割，分析和量化。研究发现，老年BAT的线粒体体积、表面积和复杂性增加，球形度降低，嵴体积、面积、周长和评分显著降低。总体而言，这些数据定义了鼠BAT中线粒体结构在衰老过程中的性质。
Mitochondria are required for energy production and even give brown adipose tissue (BAT) its characteristic color due to their high iron content and abundance. The physiological function and bioenergetic capacity of mitochondria are connected to the structure, folding, and organization of its inner-membrane cristae. During the aging process, mitochondrial dysfunction is observed, and the regulatory balance of mitochondrial dynamics is often disrupted, leading to increased mitochondrial fragmentation in aging cells. Therefore, it is hypothesized that significant morphological changes in BAT mitochondria and cristae will be present with aging. A quantitative 3D electron microscopy approach is developed to map cristae network organization in mouse BAT to test this hypothesis. Using this methodology, the 3D morphology of mitochondrial cristae is investigated in adult (3-month) and aged (2-year) murine BAT tissue via serial block face-scanning electron microscopy (SBF-SEM) and 3D reconstruction software for manual segmentation, analysis, and quantification. Upon investigation, an increase is found in mitochondrial volume, surface area, and complexity and decreased sphericity in aged BAT, alongside significant decreases in cristae volume, area, perimeter, and score. Overall, these data define the nature of the mitochondrial structure in murine BAT across aging.