CONCERTED EVOLUTION OF CLASS-I GENES IN THE MAJOR HISTOCOMPATIBILITY COMPLEX OF MURINE RODENTS
CONCERTED EVOLUTION OF CLASS-I GENES IN THE MAJOR HISTOCOMPATIBILITY COMPLEX OF MURINE RODENTS
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DOI：
10.1073/pnas.87.6.2167
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发表时间：
1990-03-01
影响因子：
11.1
通讯作者：
HOWARD, JC
中科院分区：
文献类型：
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作者：
RADA, C;LORENZI, R;HOWARD, JC
报道了褐家鼠DA菌株中两个I类主要组织相容性复合体分子的cDNA全长序列。一个编码经典I限制元素RT1。Aa，定位于大鼠主要组织相容性复合体中与小鼠H-2K同源的位点。另一个可能编码存在于DA大鼠血清中的可溶性非经典I类分子；第5外显子的短缺失意味着翻译产物将终止于跨膜区。这些序列已与小鼠经典I类序列以及三个已发表的大鼠I类cDNA部分序列进行了比较。结果表明，首先，来自H-2K、H-2D和H-2L数据集的“位点特异性”替换在RT1中被打乱。Aa分子;这些替代中的大多数具有H-2D/ l特异性特征。其次，数据显示，无论基因座或单倍型起源如何，这四个大鼠序列都惊人地相似；它们具有许多明显的物种特异性特征，这些特征使它们与小鼠经典的I类序列区别开来，后者同样具有各自独特的特征。结果表明，节段交换在ⅰ类主要组织相容性复合体分子序列进化中起主要作用。
Full-length cDNA sequences of two class I major histocompatibility complex molecules from the DA strain of Rattus norvegicus are reported. One codes for the classical I restriction element RT1.Aa, which maps to the locus in the rat major histocompatibility complex homologous to H-2K in the mouse. The other probably codes for a soluble nonclassical class I molecule present in DA rat serum; a short deletion in the fifth exon implies that the translated product will terminate in the membrane-spanning region. These sequences have been compared with mouse classical class I sequences as well as with three published rat class I cDNA partial sequences. The results show, first, that "locus-specific" substitutions from the H-2K, H-2D, and H-2L data set are scrambled in the RT1.Aa molecule; a majority of these substitutions have H-2D/L-specific features. Second, the data show that the four rat sequences are strikingly similar to one another regardless of locus or haplotype of origin; they share a number of apparently species-specific features that distinguish them all from mouse classical class I sequences, which likewise share distinctive features of their own. The results suggest that segmental exchange plays a major role in determining the evolution of sequence in class I major histocompatibility complex molecules.