Expression of miR-126 suppresses migration and invasion of colon cancer cells by targeting CXCR4
Expression of miR-126 suppresses migration and invasion of colon cancer cells by targeting CXCR4
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miR-126的表达通过靶向CXCR4抑制结肠癌细胞的迁移和侵袭
DOI：
10.1007/s11010-013-1707-6
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发表时间：
2013-09-01
影响因子：
4.3
通讯作者：
Wang, Xiaoyan
中科院分区：
文献类型：
--
作者：
Li, Zeng;Li, Nan;Wang, Xiaoyan
先前的一项研究表明，miR-126在高转移结肠癌细胞中的表达显著下调。本研究旨在探讨miR-126的生物学功能及其对结肠癌细胞靶基因的调控作用。用定量聚合酶链式反应检测结肠癌SW480和SW620细胞中miR-126的表达。用四甲基偶氮唑盐比色法检测miR-126模拟物转染后细胞活力的变化。创伤愈合和Transwell迁移侵袭实验检测肿瘤细胞在miR-126转染后对SW480和SW620细胞的迁移和侵袭能力。用荧光素酶报告基因分析和Western印迹检测miR-126靶基因(即CXCR4)的转录和表达水平。结肠癌SW480和SW620细胞中miR-126的表达水平均低于癌旁正常组织(P<；P<0.05)。与NC细胞相比，miR-126的转染组显著降低了结肠癌细胞的存活率(P<；P<；)。伤口愈合和Transwell迁移侵袭实验表明，miR-126模拟物抑制了SW480和SW620细胞的迁移和侵袭能力。生物信息学预测CXCR4是miR-126的靶基因之一。事实上，荧光素酶报告实验和Western印迹证实CXCR4是miR-126的靶基因。MiR-126的表达通过负调控CXCR4的表达，抑制了结肠癌细胞的活力，降低了肿瘤细胞的迁移和侵袭能力。
A previous study demonstrated that miR-126 expression was significantly downregulated in highly metastatic colon cancer cells. This study was to investigate the biological function of miR-126 and its regulation of target genes in colon cancer cells. Quantitative PCR was used to detect miR-126 expression in colon cancer SW480 and SW620 cells. MTT assay was to measure the changed cell viability after miR-126 mimics transfection. Wound healing and Transwell migration and invasion assays measured capacity of tumor cell migration and invasion of SW480 and SW620 cells after miR-126 transfection. Luciferase reporter assay and Western blot were used to assess both transcriptional and expression levels of one of the miR-126 target genes (i.e., CXCR4). Levels of miR-126 expression were lower in colon cancer SW480 and SW620 cells than in the adjacent normal epithelial tissues (P< 0.05). Transfection of miR-126 mimics significantly reduced colon cancer cell viability compared to NC cells (P< 0.05). The wound healing and Transwell migration and invasion assays showed that miR-126 mimics inhibited SW480 and SW620 cell migration and invasion capacity. Bioinformatics predicted that CXCR4 is one of the miR-126 target genes. Indeed, luciferase reporter assay and Western blot confirmed that CXCR4 is a miR-126 target gene. Expression of miR-126 inhibited colon cancer cell viability and reduced tumor cell migration and invasion capacity by its negative regulation of CXCR4 expression.