Function identification of MdTIR1 in apple root growth benefited from the predicted MdPPI network
Function identification of MdTIR1 in apple root growth benefited from the predicted MdPPI network
复制标题
MdTIR1在苹果根系生长中的功能识别受益于预测的MdPPI网络
DOI：
10.1111/jipb.12996
复制
发表时间：
2020-09-30
影响因子：
11.4
通讯作者：
Zhang, Shizhong
中科院分区：
文献类型：
--
作者：
Liu, Lin;Yu, Zipeng;Zhang, Shizhong
蛋白质-蛋白质相互作用（PPI）网络分析是植物发育过程中识别关键蛋白质的有效方法，特别是对苹果等缺乏基础分子研究的物种。本文在苹果中推导了包含30806个ppi的MdPPI网络，并对其质量和可靠性进行了严格验证。随后，提取根生长子网络以筛选根生长中的关键蛋白质。由于激素相关蛋白在关键蛋白中所占比例最大，因此进一步从根生长亚网络中提取激素相关子网络。在这些蛋白质中，生长素相关的m。domestic attransporsport INHIBITOR RESISTANT 1 （MdTIR1）是中心的高节点，这意味着该蛋白在根生长中发挥重要作用。此外，过表达mdtir1基因的转基因苹果根表现出了增加的主根伸长。表达分析表明，MdTIR1显著上调苹果根系生长素响应基因，表明其以生长素依赖的方式介导根系生长。进一步的实验验证表明，MdTIR1与MdIAA28、MdIAA43和MdIAA46相互作用并加速了它们的降解。因此，mdtir1介导的MdIAAs降解在苹果生长素信号转导和根系生长调控中起着至关重要的作用。此外，我们的网络分析和高节点筛选为更广泛地表征分子机制提供了一种新的研究技术。
Protein-protein interaction (PPI) network analysis is an effective method to identify key proteins during plant development, especially in species for which basic molecular research is lacking, such as apple (Malus domestica). Here, an MdPPI network containing 30 806 PPIs was inferred in apple and its quality and reliability were rigorously verified. Subsequently, a root-growth subnetwork was extracted to screen for critical proteins in root growth. Because hormone-related proteins occupied the largest proportion of critical proteins, a hormone-related sub-subnetwork was further extracted from the root-growth subnetwork. Among these proteins, auxin-relatedM. domesticaTRANSPORT INHIBITOR RESISTANT 1 (MdTIR1) served as the central, high-degree node, implying that this protein exerts essential roles in root growth. Furthermore, transgenic apple roots overexpressing anMdTIR1transgene displayed increased primary root elongation. Expression analysis showed that MdTIR1 significantly upregulated auxin-responsive genes in apple roots, indicating that it mediates root growth in an auxin-dependent manner. Further experimental validation revealed that MdTIR1 interacted with and accelerated the degradation of MdIAA28, MdIAA43, and MdIAA46. Thus, MdTIR1-mediated degradation of MdIAAs is critical in auxin signal transduction and root growth regulation in apple. Moreover, our network analysis and high-degree node screening provide a novel research technique for more generally characterizing molecular mechanisms.