Changes in microbiota during experimental human Rhinovirus infection
Changes in microbiota during experimental human Rhinovirus infection
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DOI：
10.1186/s12879-015-1081-y
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发表时间：
2015-08-14
影响因子：
3.7
通讯作者：
de Jong, M. D.
中科院分区：
文献类型：
--
作者：
Hofstra, J. J.;Matamoros, S.;de Jong, M. D.
背景：人类鼻病毒（HRV）是大多数普通感冒的罪魁祸首，并且经常伴随继发性细菌感染，其机制尚不清楚。我们研究了实验性人HRV 16血清型感染对上呼吸道微生物群的影响。方法：6名健康志愿者感染HRV16病毒。我们对感染前、感染期间和感染后采集的咽拭子进行了16S核糖体rna靶向焦磷酸测序。我们比较了不同时间点的总体群落多样性、生态系统的系统发育结构和不同细菌的相对丰度。结果：在急性感染期间，观察到副流感嗜血杆菌和亚黄奈瑟菌的相对丰度有明显的增加趋势，金黄色葡萄球菌的相对丰度有较弱的增加趋势。在第1天和第60天之间没有观察到主要差异，而受试者之间的差异非常大。结论：HRV16感染与已知与HRV感染后继发感染相关的三个属的增加有关。观察到的上呼吸道微生物群的变化有助于解释为什么HRV感染容易发生细菌性中耳炎、鼻窦炎和肺炎。
Background: Human Rhinovirus (HRV) is responsible for the majority of common colds and is frequently accompanied by secondary bacterial infections through poorly understood mechanisms. We investigated the effects of experimental human HRV serotype 16 infection on the upper respiratory tract microbiota.Methods: Six healthy volunteers were infected with HRV16. We performed 16S ribosomal RNA-targeted pyrosequencing on throat swabs taken prior, during and after infection. We compared overall community diversity, phylogenetic structure of the ecosystem and relative abundances of the different bacteria between time points.Results: During acute infection strong trends towards increases in the relative abundances of Haemophilus parainfluenzae and Neisseria subflava were observed, as well as a weaker trend towards increases of Staphylococcus aureus. No major differences were observed between day-1 and day 60, whereas differences between subjects were very high.Conclusions: HRV16 infection is associated with the increase of three genera known to be associated with secondary infections following HRV infections. The observed changes of upper respiratory tract microbiota could help explain why HRV infection predisposes to bacterial otitis media, sinusitis and pneumonia.