Axonal supercharging technique using reverse end-to-side neurorrhaphy in peripheral nerve repair: an experimental study in the rat model
Axonal supercharging technique using reverse end-to-side neurorrhaphy in peripheral nerve repair: an experimental study in the rat model
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DOI：
10.3171/jns-07/10/0821
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发表时间：
2007-10-01
影响因子：
4.1
通讯作者：
Hosmawa, Ko
中科院分区：
文献类型：
--
作者：
Fujiwara, Toshihiro;Matsuda, Ken;Hosmawa, Ko
目的。为了改善周围神经修复，在大鼠坐骨神经横断模型中研究了反向端侧神经缝合术对受损周围神经的影响。方法。将12只Sprague-Dawley大鼠分为两组（每组6只大鼠）。在第 1 组中，在臀切迹远端切断右侧坐骨神经，并使用四根 10-0 尼龙神经外膜缝合线进行端对端神经缝合术进行修复。在第I组中，进行与第I组相同的手术后，将左侧坐骨神经远端切断并通过皮下隧道到达右侧。使用 10-0 尼龙神经外膜缝合线，以端对侧的方式将左坐骨神经的近端残端与损伤点远端的右坐骨神经的神经外膜窗接合。使用后爪镇痛仪记录、电生理测试、肌肉收缩力测量、双标记技术、腓肠肌重量测量和组织学检查、双侧坐骨神经组织学检查和免疫荧光染色来评估效果。用于评估反向端侧神经缝合技术的许多测试结果表明，轴突增强促进了去神经靶器官的功能恢复。结论。反向端侧神经缝合技术可用于周围神经修复。
Object. In an attempt to improve peripheral nerve repair, the influence of the addition of reverse end-to-side neurorrhaphy for an injured peripheral nerve was investigated in the rat sciatic nerve transection model.Methods. Twelve Sprague-Dawley rats were divided into two groups (six rats in each group). In Group 1, the right sciatic nerve was cut at a point distal to the gluteal notch and repaired using end-to-end neurorrhaphy with four 10-0 nylon epineurial sutures. In Group It, after performing the same procedure as in Group I, the left sciatic nerve was cut distally and passed through a subcutaneous tunnel to the right side. The proximal stump of the left sciatic nerve was co-apted to the epineurial window of the right sciatic nerve distal to the injured point in an end-to-side fashion using 10-0 nylon epineurial sutures. The effects were evaluated using analgesimeter recordings for the hind paw, electrophysiological tests, measurement of the muscle contraction force, a double-labeling technique, weight measurement and histological examination of the gastrocnemius muscle, histological examination of the bilateral sciatic nerves, and immunofluorescent staining.Results. Results from the many tests used to evaluate the reverse end-to-side neurorrhaphy technique indicated that functional recovery of the denervated target organs was promoted by axonal augmentation.Conclusions. The reverse end-to-side neurorrhaphy technique could be useful in peripheral nerve repair.