Synthesis of a heparin-related GlcN-IdoA sulfation-site variable disaccharide library and analysis by Raman and ROA spectroscopy.
Synthesis of a heparin-related GlcN-IdoA sulfation-site variable disaccharide library and analysis by Raman and ROA spectroscopy.
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DOI：
10.1016/j.carres.2014.06.026
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发表时间：
2014-12-05
影响因子：
3.1
通讯作者：
Gardiner, John M.
中科院分区：
文献类型：
--
作者：
Miller, Gavin J.;Hansen, Steen U.;Barath, Marek;Johannessen, Christian;Blanch, Ewan W.;Jayson, Gordon C.;Gardiner, John M.
硫酸化程序化GlcN-IdoA二糖基质的合成。证明了使用各种GlcN供体和Ido受体的有效合成。四种均匀的硫酸化变化的肝素二糖用于获得拉曼和拉曼罗阿数据。光谱提供了不同硫酸化水平的特征硫酸化条带的指示性比较。数据用于提供与天然肝素光谱的比较/差异。描述了直接从l-艾杜糖醛酸组分合成一系列差异硫酸化GlcN-IdoA二糖，其可在良好规模上获得。这些特别针对在该肝素二糖的关键的最常见的生物学相关的硫酸化可变性I-IdoA O-2和d-GlcN O-6和氨基位点处提供硫酸化可变性。这种硫酸化变化的基质允许使用拉曼/罗阿光谱的第一次评估，以表征光谱的变化作为纯的，定义的肝素相关二糖物质的硫酸化位点和水平的函数。这提供了对消化天然肝素的相似性和差异的分析，并且这显示了作为二糖水平上某些特定硫酸化变化的函数的构象和构象自由度的不同类型变化的证据。预计该数据集将为进一步的位点特异性硫酸化修饰的应用开辟道路，并证明Raman-ROA对肝素片段中的硫酸化进行指纹识别的能力。
Synthesis of a matrix of sulfation-programmed GlcN–IdoA disaccharides. Demonstrates effective synthesis using various GlcN donors and Ido acceptor. Four homogeneous sulfation-varied heparin disaccharides used to obtain Raman and Raman ROA data. Spectra provide indicative comparisons of signature sulfation bands for differing sulfation levels. Data are used to provide comparisons/differences to native heparin spectra. Synthesis of an array of differentially sulfated GlcN–IdoA disaccharides, accessible on good scale, directly from l-iduronate components is described. These are specifically directed to provide the sulfation variability at the key most common biologically relevant sulfation-variable l-IdoA O-2 and d-GlcN O-6 and amino sites of this heparin disaccharide. This sulfation-varied matrix has allowed the first evaluation of using Raman/ROA spectroscopy to characterize changes in spectra as a function of both site and level of sulfation with pure, defined heparin-related disaccharide species. This provides analysis of both similarities and differences to digest native heparin and this shows evidence of different types of changes in conformations and conformational freedom as a function of some specific sulfation changes at the disaccharide level. It is anticipated that this data set will open the way for applications to further site-specific sulfated saccharides and demonstrates the capability offered by Raman–ROA towards fingerprinting sulfation in heparin fragments.
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影响因子：
15
作者：
FERRO, DR;PROVASOLI, A;CHOAY, J
通讯作者：
CHOAY, J
影响因子：
6.9
作者：
Casu, Benito;Naggi, Annamaria;Torri, Giangiacomo
通讯作者：
Torri, Giangiacomo
影响因子：
4
作者：
de Paz, Lose L.;Moseman, E. Ashley;Seeberger, Peter H.
通讯作者：
Seeberger, Peter H.
影响因子：
15
作者：
BELL, AF;HECHT, L;BARRON, LD
通讯作者：
BARRON, LD
影响因子：
14.8
作者：
Gama, Cristal I.;Tully, Sarah E.;Hsieh-Wilson, Linda C.
通讯作者：
Hsieh-Wilson, Linda C.