Combining DNA expression with positional information to detect functional silencing of chromosomal regions
Combining DNA expression with positional information to detect functional silencing of chromosomal regions
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DOI：
10.1093/bioinformatics/btg314
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发表时间：
2003-12-12
期刊：
影响因子：
5.8
通讯作者：
Jöckel, KH
中科院分区：
文献类型：
--
作者：
Hüsing, J;Zeschnigk, M;Jöckel, KH
动机：在癌细胞中经常观察到染色体物质的丢失。在这种情况下，基因的表达与它们在基因组中的位置有关。表观遗传现象也可能使染色体同一区域的几个基因沉默。虽然细胞遗传学或其他分子遗传学方法现货的DNA拷贝数的变化，他们不能检测到其他原因silencing.Results：我们提出了一种方法，利用从高密度DNA微阵列获得的基因位点的表达信息的链接，根据目前的数据库。统计方法足以当场显着运行的非表达基因的介绍和相互比较的优点，他们的权力和鲁棒性对假阳性。
Motivation: Loss of chromosomal material is often observed in cancer cells. In this situation the expression of genes is related to their position on the genome. Epigenetic phenomena may also silence several genes in the same region of a chromosome. While cytogenetic or other molecular genetic methods spot changes of DNA copy number, they cannot detect other causes of silencing.Results: We propose a method that utilizes the link from expression information gained from high-density DNA microarrays to the gene locus according to current databases. Statistical methods adequate to spot conspicuous runs of non-expressed genes are introduced and compared to one another by merit of their power and robustness against false positives.