Smart Self-Assembled Organic Nanoprobe for Protein-Specific Detection: Design, Synthesis, Application, and Mechanism Studies
Smart Self-Assembled Organic Nanoprobe for Protein-Specific Detection: Design, Synthesis, Application, and Mechanism Studies
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用于蛋白质特异性检测的智能自组装有机纳米探针：设计、合成、应用和机制研究
DOI：
10.1021/acs.analchem.7b02923
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发表时间：
2017-09-19
影响因子：
7.4
通讯作者：
Cheng, Zhen
中科院分区：
文献类型：
--
作者：
Gao, Tang;Yang, Shuqi;Cheng, Zhen
特异性检测或成像蛋白质具有很大的潜力，可以为医学诊断、生物研究和治疗应用做出巨大贡献。血液中人血清白蛋白（HSA）的水平与多种疾病相关，因此可以作为临床快速诊断的重要生物标志物。在这里，我们报告了使用基于聚集诱导发射（AIE）的超分子组装来设计用于检测蛋白质 HSA 的生物分子响应智能有机纳米材料。设计的纳米探针是小分子的聚集体，荧光沉默，但在 HSA 存在的情况下，它们会分解并产生清晰的开启荧光信号。在为 HSA 检测而构建的小型纳米探针库中，结构光学信号传导和筛选研究表明，纳米探针 7 是最有效的。机理研究表明，纳米探针7通过多种非共价相互作用与HSA的位点I结合。由此产生的纳米探针 7 在 HSA 疏水腔中分子内旋转的限制诱导荧光发射，这通过竞争性结合测定和分子对接得到了验证。更重要的是，nanoprobe 7成功应用于人血清样本中HSA的识别和定量。这项研究表明，纳米探针7是临床真实、快速检测HSA的一种很有前景的工具，因此可能会找到许多应用，而基于AIE的分子组装也为设计智能纳米材料以灵敏、选择性地检测各种分析物开辟了一条新途径。
Specific detection or imaging protein has high potential to contribute,greatly to medical diagnosis, biological research, and therapeutic applications. The level of human serum albumin (HSA) in blood is related to a variety of diseases and thus serves as an important biomarker for fast clinical diagnosis. Here we report the use of aggregation-induced emission (AIE) based supramolecular assembly to design biomolecular responsive smart organic nanomaterials for detection protein HSA. The designed nanoprobes were aggregates of small molecules and silent in fluorescence, but in the presence of HSA they disassembled and produced a clear turn-on fluorescent signal. Of a small library of nanoprobes constructed for HSA detection, structure-optical signaling and screening studies revealed that nanoprobe 7 is the most efficient one. Mechanism studies showed that nanoprobe 7 was bonded with Site I of HSA through the multiple noncovalent interactions. The resultant restriction of intramolecular rotation of nanoprobe 7 in the hydrophobic cavity of HSA induced fluorescent emission, which was validated by competitive binding assays and molecular docking. More importantly, nanoprobe 7 was successfully applied to recognize and quantify HSA in human serum samples. This study demonstrates nanoprobe 7 is a promising tool for clinical real and fast detection of HSA and thus may find many applications, and the molecular assembly based on AIE also opens a new avenue for designing smart nanomaterials for the sensitive and selective detection for varied analytes.