Hibernation induces widespread transcriptional remodeling in metabolic tissues of the grizzly bear
Hibernation induces widespread transcriptional remodeling in metabolic tissues of the grizzly bear
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DOI：
10.1038/s42003-019-0574-4
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发表时间：
2019-09-13
影响因子：
5.9
通讯作者：
Kelley, Joanna L.
中科院分区：
文献类型：
--
作者：
Janseng, Heiko T.;Trojahn, Shawn;Kelley, Joanna L.
揭示冬眠的可逆生理机制可能对人类和动物健康都有应用，因为冬眠通常与疾病状态有关。本研究利用RNA测序技术揭示了灰熊（Ursus arctos horribilis）的组织和季节性转录变化。与其他季节相比，冬眠时熊脂肪中差异表达的基因比肝脏和骨骼肌中的差异表达基因要多。在摄食过多期间，脂肪与活跃季节相比有超过900个差异表达基因。冬眠的特征在于与胰岛素信号传导、肌肉蛋白降解和尿素产生相关的基因的表达减少，以及肌肉蛋白合成代谢途径内的表达增加。在所有三种组织中，我们发现了一个共享的差异表达基因的子集，其中一些是未知的，它们共同反映了一种共同的调控机制。所鉴定的基因家族可用于开发治疗人类和动物疾病的新疗法。
Revealing the mechanisms underlying the reversible physiology of hibernation could have applications to both human and animal health as hibernation is often associated with disease-like states. The present study uses RNA-sequencing to reveal the tissue and seasonal transcriptional changes occurring in grizzly bears (Ursus arctos horribilis). Comparing hibernation to other seasons, bear adipose has a greater number of differentially expressed genes than liver and skeletal muscle. During hyperphagia, adipose has more than 900 differentially expressed genes compared to active season. Hibernation is characterized by reduced expression of genes associated with insulin signaling, muscle protein degradation, and urea production, and increased expression within muscle protein anabolic pathways. Across all three tissues we find a subset of shared differentially expressed genes, some of which are uncharacterized, that together may reflect a common regulatory mechanism. The identified gene families could be useful for developing novel therapeutics to treat human and animal diseases.