Antenna holochrome B880 of Rhodospirillum rubrum S1. Pigment, phospholipid, and polypeptide composition
Antenna holochrome B880 of Rhodospirillum rubrum S1. Pigment, phospholipid, and polypeptide composition
复制标题
红色红螺菌 S1 的天线全息 B880。
DOI：
10.1021/bi00279a028
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
G. Gingras
中科院分区：
文献类型：
--
作者：
R. Castaño;G. Bélanger;G. Gingras
Rafael Picorel，Gilles Belanger和Gabriel Gingras* 摘要：从红杜鹃的色素体中分离出一种天线细菌叶绿素蛋白复合物（全色素B880）。以全色素形式回收的色素体细菌叶绿素的产率在初始粗级分中约为90%，在两个色谱纯化步骤后约为75%。全色素蛋白估计约占总色素蛋白的60%。根据十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，该制剂含有两种不同的多肽，分别命名为a和，表观Mr分别为7600和6400。很可能，多肽a是在其他实验室中较早分离和测序的有机溶剂可溶性多肽。根据聚丙烯酰胺凝胶中考马斯亮蓝染色的相对强度，多肽a和/3以等摩尔比存在。氨基
Rafael Picorel, Gilles Belanger, and Gabriel Gingras* abstract: An antenna bacteriochlorophyll-protein complex (holochrome B880) was isolated from chromatophores of Rhodospirillum rubrum. The yield of chromatophore bac-teriochlorophyll recovered as holochrome was about 90% in the initial crude fractions and about 75% after two chromatographic purification steps. The holochrome protein is es-timated to account for approximately 60% of the total chromatophore proteins. According to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, this preparation contains two different polypeptides, designated a and of apparent Mr 7600 and 6400. Very likely, polypeptide a is the organic solvent soluble polypeptide earlier isolated and sequenced in other laboratories. According to their relative intensity of staining by Coomassie Brilliant Blue in polyacrylamide gels, polypeptides a and/3 are present in an equimolar ratio. Amino