Stimulation of rat pancreatic exocrine secretion by cocaine- and amphetamine-regulated transcript peptide
Stimulation of rat pancreatic exocrine secretion by cocaine- and amphetamine-regulated transcript peptide
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DOI：
10.1016/s0167-0115(01)00226-9
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发表时间：
2001-05-05
影响因子：
--
通讯作者：
Mulholland, MW
中科院分区：
文献类型：
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作者：
Cowles, RA;Segura, BJ;Mulholland, MW
可卡因和安非他明调节转录 (CART) 肽是最近描述的一种神经肽，已定位于中枢和周围神经系统区域。 CART 已被证明在中央注射时参与摄食行为，但其对外周组织的影响尚未研究。该报告描述了CART肽对大鼠胰腺外分泌分泌的影响。注射CART肽后，用胆胰管插管制成的麻醉大鼠的淀粉酶分泌量增加了四倍。 CART 肽诱导的胰腺分泌增加似乎涉及对乙酰胆碱 (ACh) 和缩胆囊素 (CCK) 敏感的途径，因为阿托品（ACh 受体拮抗剂）或 L-364,718（CCK-A 受体拮抗剂）预处理抑制了 CART 肽对淀粉酶分泌的影响。阿托品和 L-364,718 组合的预处理消除了 CART 肽的作用。当分离的大鼠胰腺腺泡暴露于不同剂量的 CART 肽时，没有观察到淀粉酶分泌增加。本研究结果表明，CART肽对胰腺外分泌分泌具有刺激作用。 CART 肽诱导的胰腺分泌增加似乎是间接介导的，因为没有显示对胰腺腺泡的直接影响。 CART 肽可能作用于远离腺泡单位的胰腺分泌功能的外周或中枢调节器。 (C) 2001 年由 Elsevier Science B.V. 出版
Cocaine- and amphetamine-regulated transcript (CART) peptide is a recently described neuropeptide that has been localized to areas of the central and peripheral nervous systems. CART has been shown to be involved in feeding behavior when injected centrally, however, its effects upon peripheral tissues have not been studied. This report describes the effects of CART peptide on rat pancreatic exocrine secretion. Infusion of CART peptide caused four-fold increases in amylase secretion from anesthetized rats that had been fashioned with a bile-pancreatic duct cannula. CART peptide-induced increases in pancreatic secretion appear to involve pathways that are sensitive to both acetylcholine (ACh) and cholecystokinin (CCK) since pre-treatment with atropine (ACh receptor antagonist) or L-364,718 (CCK-A receptor antagonist) inhibited the effects of CART peptide on amylase secretion. Pre-treatment with a combination of atropine and L-364,718 abolished the effects of CART peptide. When isolated rat pancreatic acini were exposed to varying doses of CART peptide, no increase in amylase secretion was observed. The results of the present study suggest that CART peptide has stimulatory effects upon pancreatic exocrine secretion. CART peptide-induced increases in pancreatic secretion appear to be indirectly mediated as no direct effect upon pancreatic acini was shown. CART peptide likely acts upon either peripheral or central regulators of pancreatic secretory function that are distant from the acinar unit. (C) 2001 Published by Elsevier Science B.V.