COMMON MECHANISTIC ACTION OF BACTERIOCINS FROM LACTIC-ACID BACTERIA
COMMON MECHANISTIC ACTION OF BACTERIOCINS FROM LACTIC-ACID BACTERIA
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DOI：
10.1128/aem.59.9.3003-3010.1993
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发表时间：
1993-09-01
影响因子：
4.4
通讯作者：
MONTVILLE, TJ
中科院分区：
文献类型：
--
作者：
BRUNO, MEC;MONTVILLE, TJ
研究了4种乳酸菌细菌素对敏感生物质子动力学的影响。Pediocin PA-1（20 μ g/ml）和leuconocin S（48.5 μ g/ml）以浓度依赖性方式介导了能量化单核细胞增生李斯特菌Scott A细胞的全部或主要PMF耗散，如乳链菌肽所示。乳酸菌素F（13.5 μ g/ml）导致激发的德氏乳杆菌ATCC 4797细胞的PMF消耗87%，并且也呈浓度依赖性。通过使用离子载体尼日利亚菌素和缬氨霉素对靶生物进行部分和特异性的除菌，测定了这四种细菌素活性所需的能量。Pediocin PA-1，leuconocin S和lactacin F以能量不依赖的方式起作用，而nisin的活性被证实是能量依赖的。这些结果与已发表的其他细菌素的报告表明，乳酸菌的细菌素有一个共同的机制，PMF的消耗。
The influence of four bacteriocins from lactic acid bacteria on the proton motive force (PMF) of sensitive organisms was investigated. Pediocin PA-1 (20 mug/ml) and leuconocin S (48.5 mug/ml) mediated total or major PMF dissipation of energized Listeria monocytogenes Scott A cells in a concentration-dependent manner, as has been shown for nisin. Lactacin F (13.5 mug/ml) caused 87% PMF depletion of energized Lactobacillus delbrueckii ATCC 4797 cells, also in a concentration-dependent fashion. The energy requirements for the activity of these four bacteriocins were determined by using the ionophores nigericin and valinomycin to carry out partial and specific deenergization of the target organisms. Pediocin PA-1, leuconocin S, and lactacin F acted in an energy-independent manner, whereas the activity of nisin was confirmed to be energy dependent. These results together with published reports on other bacteriocins suggest that the bacteriocins of lactic acid bacteria share a common mechanism, the depletion of PMF.