Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans
Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans
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DOI：
10.1016/j.intimp.2006.04.002
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发表时间：
2006-08-01
影响因子：
5.6
通讯作者：
Maiti, Tapas K.
中科院分区：
文献类型：
--
作者：
Sarangi, Itisam;Ghosh, Dipanjan;Maiti, Tapas K.
平菇是广泛栽培的食用菌之一。通过醇沉、离子交换和凝胶渗透色谱（Sephadex G-100）纯化糙皮侧耳菌丝体水溶性蛋白多糖组分。发现三个中性级分，其多糖与蛋白质的比例分别为14.2，26.4和18.3。在荷肉瘤-180小鼠模型上测试这些级分的体外和体内免疫调节和抗癌作用。体内注射蛋白多糖可减少荷肉瘤180小鼠的肿瘤细胞数量，细胞周期分析显示，大多数细胞被阻滞在细胞周期的pre-G（0）/G（1）期。所有的三种蛋白聚糖升高小鼠自然杀伤（NK）细胞的细胞毒性和刺激巨噬细胞产生一氧化氮。傅里叶变换红外光谱（FTIR）表明，β-糖苷键的存在下，在所有的馏分。组分I与葡萄糖/甘露糖特异性凝集素伴刀豆球蛋白A（ConA）强烈相互作用，表明存在大量具有葡萄糖/甘露糖的末端糖。因此，这三种中性蛋白多糖可作为免疫调节剂和抗癌剂。(c)2006 Elsevier B. V.保留所有权利。
Pleurotus ostreatits is one of the widely cultivated edible mushrooms. Water-soluble proteoglycan fractions from P. ostreatus mycelia were purified by alcohol-precipitation, ion exchange and followed by gel permeation (Sephadex G-100) chromatography. Three neutral fractions were found, which had polysaccharide to protein ratios 14.2, 26.4 and 18.3, respectively. These fractions were tested for in vitro and in vivo immunomodulatory and anticancer effects on Sarcoma-180-bearing mouse model. In vivo injection of proteoglycans to Sarcoma-180-bearing mice decreased the number of tumor cells and cell cycle analysis showed that most of the cells were found to be arrested in pre-G(0)/G(1) phase of cell cycle. All of the three proteoglycans elevated mouse natural killer (NK) cell cytotoxicity and stimulated macrophages to produce nitric oxide. The Fourier transform infra red (FTIR) spectra suggested the presence of beta-glycosidic bond in all the fractions. Fraction I strongly interacted with glucose/mannose-specific lectin Concanavalin A (ConA), indicating the presence of large number of terminal sugar with glucose/mannose. Thus, the three neutral proteoglycans derived from the mushroom (P. ostreatus) mycelia could be used as immunomodulators and anti cancer agents. (c) 2006 Elsevier B.V. All rights reserved.