Patch-clamp studies of isolated mouse olfactory receptor neurons.
Patch-clamp studies of isolated mouse olfactory receptor neurons.
复制标题
DOI：
10.1085/jgp.90.1.95
复制
发表时间：
1987-07
期刊：
影响因子：
--
通讯作者：
Dionne VE
中科院分区：
文献类型：
--
作者：
Maue RA;Dionne VE
用膜片钳技术研究了从胚胎、新生和成年小鼠中分离的嗅觉受体神经元。在神经元索马和受体神经元的树突状突起的膜斑中发现了几种不同类型的离子通道，包括130 pS Ca++激活的电压依赖性K+通道，80 pS Ca++激活的电压不敏感性K+通道，25 pS的内向整流器K+通道，和40 pS的K+通道，其被激活，然后通过快速去极化而失活。大电导（大于200 pS）的Cl-通道，这是Ca++不敏感和越来越活跃的去极化膜电位，电压激活的Ca++通道（16 pS）的证据也得到了。根据从细胞附着的贴片记录的K+通道活性，推断细胞内[Ca++]低于0.1 μ M，并且推断膜电位约为-50 mV。受体神经元具有高的输入电阻，皮安量的去极化电流可诱发动作电位。受体神经元响应应用气味分子和毛喉素与膜电导的增加。这些结果提供了一个描述的嗅觉受体神经元的膜特性和了解他们的电活动和对气味的反应的基础。
Olfactory receptor neurons isolated from embryonic, neonatal, and adult mice were studied using the patch-clamp technique. Several distinct types of ion channels were characterized in patches of membrane from the neuronal soma and the dendritic knob of receptor neurons, including a 130-pS Ca++-activated K+ channel with voltage-dependent kinetics, an 80-pS Ca++-activated K+ channel with voltage-insensitive kinetics, a 25- pS K+ channel with properties similar to inward rectifiers, and a 40-pS K+ channel that was activated and then inactivated by rapid depolarization. Evidence of large-conductance (greater than 200 pS) Cl- channels, which were Ca++ insensitive and increasingly active at depolarizing membrane potentials, and voltage-activated Ca++ channels (16 pS) was also obtained. From K+ channel activity recorded from cell- attached patches, the intracellular [Ca++] was inferred to be below 0.1 microM, and the membrane potential was inferred to be approximately -50 mV. The receptor neurons had high input resistances, and action potentials could be elicited by picoampere amounts of depolarizing current. The receptor neurons responded to applied odorant molecules and to forskolin with increases in membrane conductance. These results provide a description of the membrane properties of olfactory receptor neurons and a basis for understanding their electrical activity and response to odorants.