Large-scale proteome analysis of abscisic acid and ABSCISIC ACID INSENSITIVE3-dependent proteins related to desiccation tolerance in Physcomitrella patens.
Large-scale proteome analysis of abscisic acid and ABSCISIC ACID INSENSITIVE3-dependent proteins related to desiccation tolerance in Physcomitrella patens.
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DOI：
10.1016/j.bbrc.2016.02.024
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发表时间：
2016-03
影响因子：
3.1
通讯作者：
Izumi Yotsui;S. Serada;T. Naka;Masashi Saruhashi;T. Taji;Takahisa Hayashi;R. Quatrano;Y. Sakata
中科院分区：
文献类型：
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作者：
Izumi Yotsui;S. Serada;T. Naka;Masashi Saruhashi;T. Taji;Takahisa Hayashi;R. Quatrano;Y. Sakata
耐旱性是陆地植物的祖先特征，并且仍然保留在非维管植物如britectes和一些维管植物中。然而，除了种子和孢子外，这种性状在维管植物的营养组织中不存在。虽然许多研究都集中在利用转录组和蛋白质组的方法来了解脱水耐受性的分子基础，但脱水耐受植物和非脱水植物之间的关键分子差异仍然不清楚。在实验室条件下，藓类植物立碗藓不能在快速干燥中存活，但如果在干燥前用植物激素脱落酸（阿坝）处理原丝细胞，它可以在干燥条件下存活24小时，并在再水化后再生。阿坝（AiDT）诱导的脱水耐受性是该激素所特有的，但也依赖于植物转录因子脱落酸不敏感3（ABI3）。本文报道了利用iTRAQ（IsobaricTagsforRelative andAbsoluteQuantification）技术对帕氏原杆菌野生型和ABI3缺失突变体（Δabi3）AiDT进行比较蛋白质组学分析。从我们鉴定的总共1980个独特的蛋白质中，只有16个蛋白质在阿坝处理后干燥后与野生型相比在Δ abi3中显著改变。在这一组中，在Δ abi3组织中受到严重影响的四种蛋白质中有三种是拟南芥直向同源基因，它们在成熟种子中表达，受ABI3的调控。这些包括第1组晚期胚胎发育丰富（莱亚）蛋白，短链脱氢酶，和脱水相关蛋白。我们的研究结果表明，至少有三个这些蛋白表达在干旱耐受细胞的拟南芥和苔藓很可能发挥重要作用，在陆地植物的干旱耐受性的收购。此外，我们的研究结果表明，阿坝和ABI3介导的基因表达的干旱耐受性的调节机制可能已经在祖先的陆地植物分离前brabrites和维管植物。
Desiccation tolerance is an ancestral feature of land plants and is still retained in non-vascular plants such as bryophytes and some vascular plants. However, except for seeds and spores, this trait is absent in vegetative tissues of vascular plants. Although many studies have focused on understanding the molecular basis underlying desiccation tolerance using transcriptome and proteome approaches, the critical molecular differences between desiccation tolerant plants and non-desiccation plants are still not clear. The mossPhyscomitrella patenscannot survive rapid desiccation under laboratory conditions, but if cells of the protonemata are treated by the phytohormone abscisic acid (ABA) prior to desiccation, it can survive 24 h exposure to desiccation and regrow after rehydration. The desiccation tolerance induced by ABA (AiDT) is specific to this hormone, but also depends on a plant transcription factor ABSCISIC ACID INSENSITIVE3 (ABI3). Here we report the comparative proteomic analysis of AiDT between wild type and ABI3 deleted mutant (Δabi3) ofP. patensusing iTRAQ (IsobaricTags forRelative andAbsoluteQuantification). From a total of 1980 unique proteins that we identified, only 16 proteins are significantly altered inΔabi3compared to wild type after desiccation following ABA treatment. Among this group, three of the four proteins that were severely affected inΔabi3tissue were Arabidopsis orthologous genes, which were expressed in maturing seeds under the regulation of ABI3. These included a Group 1 late embryogenesis abundant (LEA) protein, a short-chain dehydrogenase, and a desiccation-related protein. Our results suggest that at least three of these proteins expressed in desiccation tolerant cells of both Arabidopsis and the moss are very likely to play important roles in acquisition of desiccation tolerance in land plants. Furthermore, our results suggest that the regulatory machinery of ABA- and ABI3-mediated gene expression for desiccation tolerance might have evolved in ancestral land plants before the separation of bryophytes and vascular plants.