Graphene oxide-modified 3D acellular cartilage extracellular matrix scaffold for cartilage regeneration
Graphene oxide-modified 3D acellular cartilage extracellular matrix scaffold for cartilage regeneration
复制标题
氧化石墨烯修饰的 3D 无细胞软骨细胞外基质支架用于软骨再生。
DOI：
10.1016/j.msec.2020.111603
复制
发表时间：
2021-02-01
影响因子：
7.9
通讯作者：
Xiang, Zhou
中科院分区：
文献类型：
--
作者：
Gong, Min;Sun, Jiachen;Xiang, Zhou
关节软骨再生是骨科和组织工程领域的一个挑战。本研究制备了氧化石墨烯（GO）修饰的三维脱细胞软骨细胞外基质（ACM）支架用于软骨修复。软骨切片的脱细胞使用物理和化学方法制造ACM颗粒的组合。用1-乙基-3-（3-二甲氨基丙基）盐酸碳二亚胺和n -羟基琥珀酰亚胺与氧化石墨烯交联制备复合支架。GO改性改善了支架的内部结构和力学性能。氧化石墨烯修饰的复合支架（2mg /mL）可促进体外细胞粘附、细胞增殖和软骨分化。大鼠皮下植入实验表明，复合支架具有良好的生物相容性和轻微的炎症反应。植入12周后，负载骨髓间充质干细胞的复合支架以透明软骨完全桥接兔膝关节软骨缺损。结果表明，go修饰的三维ACM复合支架可为软骨组织工程和关节软骨损伤治疗提供强大的平台。
Articular cartilage regeneration is a challenge in orthopedics and tissue engineering. This study prepared a graphene oxide (GO)-modified 3D acellular cartilage extracellular matrix (ACM) scaffold for cartilage repair. Cartilage slices were decellularized using a combination of physical and chemical methods of fabricating ACM particles. GO was crosslinked with the ACM by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy succinimide to prepare a composite scaffold. GO modification improved the internal structure and mechanical properties of the scaffold. The GO-modified (2 mg/mL) composite scaffold promoted cell adhesion, cell proliferation, and chondrogenic differentiation in vitro. Experiments on subcutaneous implantation in rats demonstrated that the composite scaffold had good biocompatibility and mild inflammatory response. After 12 weeks of implantation, the composite scaffold loaded with bone marrow mesenchymal stem cells completely bridged the cartilage defects in the rabbit knee with hyaline cartilage. Results indicated that the GO-modified 3D ACM composite scaffold can provide a powerful platform for cartilage tissue engineering and articular cartilage injury treatment.