Emergence of Fibrocytes Showing Morphological Changes in the Inflamed Colonic Mucosa
Emergence of Fibrocytes Showing Morphological Changes in the Inflamed Colonic Mucosa
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DOI：
10.1007/s10620-009-0730-7
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发表时间：
2010-02-01
影响因子：
3.1
通讯作者：
Saito, Yasushi
中科院分区：
文献类型：
--
作者：
Uehara, Hirotsugu;Nakagawa, Tomoo;Saito, Yasushi
纤维细胞有助于伤口愈合，并且是由造血细胞和间充质细胞标记物的共同表达而独特定义的。在这项研究中，在给予3%葡聚糖硫酸钠（DSS） 7天诱导的小鼠结肠炎模型中测定纤维细胞的运输。结肠组织免疫染色检测CD45、I型胶原（Col I）和α - sma。第0天，结肠组织中没有CD45(+) Col I+细胞。而在炎症细胞明显积聚的第7天，粘膜下层出现了明显的卵形CD45(+) Col I+纤维细胞。第14天，结肠组织处于愈合期，可见大量梭形CD45(+) Col I+纤维细胞。CD45(+) Col I+纤维细胞的出现先于α - sma（+）肌成纤维细胞的出现。早在结肠炎炎症期募集的卵形纤维细胞很可能在愈合期分化为梭形纤维细胞，提示纤维细胞可能促进炎症结肠组织的伤口愈合。
Fibrocytes contribute to wound healing and are uniquely defined by coexpression of hematopoietic and mesenchymal cell markers. In this study, trafficking of fibrocytes was determined in a murine model of colitis induced by administering 3% dextran sodium sulfate (DSS) for seven days. Colonic tissues were immunostained for CD45, collagen type I (Col I), and alpha-SMA. On day 0, there were no CD45(+) Col I+ cells in colonic tissues. However, on day 7 when inflammatory cells showed remarkable accumulation, oval-shaped CD45(+) Col I+ fibrocytes were obvious in the submucosal layer. On day 14 when colonic tissues were in the healing phase, numerous spindle-shaped CD45(+) Col I+ fibrocytes were observed. Emergence of CD45(+) Col I+ fibrocytes preceded the appearance of alpha-SMA(+) myofibroblasts. Oval-shaped fibrocytes recruited as early as the inflammatory phase of colitis are likely to differentiate into spindle-shaped fibrocytes in the healing phase, suggesting that fibrocytes may promote wound healing in inflamed colonic tissues.