Exposure to Deepwater Horizon Crude Oil Burnoff Particulate Matter Induces Pulmonary Inflammation and Alters Adaptive Immune Response.
Exposure to Deepwater Horizon Crude Oil Burnoff Particulate Matter Induces Pulmonary Inflammation and Alters Adaptive Immune Response.
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DOI：
10.1021/acs.est.5b01439
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发表时间：
2015-07-21
影响因子：
11.4
通讯作者：
Cormier SA
中科院分区：
文献类型：
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作者：
Jaligama S;Chen Z;Saravia J;Yadav N;Lomnicki SM;Dugas TR;Cormier SA
2010年“深水地平线”（DWH）漏油事件中，墨西哥湾沃茨表层原油的“原位燃烧”释放出大量污染物，其中包括燃烧产生的颗粒物（PM）。关于吸入现场燃烧的油帆颗粒物（OSPM）的呼吸影响的信息有限。在这里，我们利用从DWH石油泄漏现场燃烧羽流收集的PM来研究暴露于OSPM对肺部健康的急性影响。OSPM在体外引起剂量和时间依赖性的细胞毒性，并产生活性氧和超氧自由基。此外，暴露于OSPM的小鼠表现出体重增加显著降低，血清8-异前列烷（8-IP）水平增加形式的全身氧化应激，以及支气管肺泡灌洗液中巨噬细胞和嗜酸性粒细胞增加形式的气道炎症。此外，在过敏性哮喘的小鼠模型中，OSPM引起辅助性T细胞2（Th 2）增加、细支气管周围炎症和气道粘液产生增加。这些发现表明，急性暴露于OSPM导致肺部炎症和小鼠先天/适应性免疫反应的改变，并突出了与清理漏油相关的潜在呼吸影响。
The ″in situ burning” of trapped crude oil on the surface of Gulf waters during the 2010 Deepwater Horizon (DWH) oil spill released numerous pollutants, including combustion-generated particulate matter (PM). Limited information is available on the respiratory impact of inhaled in situ burned oil sail particulate matter (OSPM). Here we utilized PM collected from in situ burn plumes of the DWH oil spill to study the acute effects of exposure to OSPM on pulmonary health. OSPM caused dose-and time-dependent cytotoxicity and generated reactive oxygen species and superoxide radicals in vitro. Additionally, mice exposed to OSPM exhibited significant decreases in body weight gain, systemic oxidative stress in the form of increased serum 8-isoprostane (8-IP) levels, and airway inflammation in the form of increased macrophages and eosinophils in bronchoalveolar lavage fluid. Further, in a mouse model of allergic asthma, OSPM caused increased T helper 2 cells (Th2), peribronchiolar inflammation, and increased airway mucus production. These findings demonstrate that acute exposure to OSPM results in pulmonary inflammation and alteration of innate/adaptive immune responses in mice and highlight potential respiratory effects associated with cleaning up an oil spill.