In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells.
In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells.
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DOI：
10.1038/s41377-021-00542-5
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发表时间：
2021-05-28
期刊：
影响因子：
--
通讯作者：
Wei X
中科院分区：
文献类型：
--
作者：
Zhu X;Suo Y;Fu Y;Zhang F;Ding N;Pang K;Xie C;Weng X;Tian M;He H;Wei X
循环肿瘤细胞（CTC）是癌症转移的既定生物标志物。 CTC 的循环动态对于理解肿瘤细胞传播的机制非常重要。尽管研究表明昼夜节律可能会扰乱肿瘤的生长，但目前尚不清楚昼夜节律是否控制CTC的释放。在临床检查中，目前检测血液样本中CTC的体外方法基于一个基本假设，即外周血中CTC计数不会随时间发生显着变化，这一点受到最近研究的挑战。由于重复从患者身上抽血是不切实际的，因此研究 CTC 昼夜节律的一个可行策略是通过体内检测方法对其进行监测。荧光体内流式细胞术（IVFC）是一种强大的光学技术，能够以无创方式长期直接检测活体动物中的荧光循环细胞。在这项研究中，我们应用荧光 IVFC 在人类前列腺癌原位小鼠模型中无创监测 CTC。我们观察到 CTC 在癌症进展过程中表现出随机爆发。早期爆发活动的概率高于后期。我们在 24 小时内纵向监测 CTC，结果显示 CTC 计数存在显着的每日波动，并在夜间（啮齿类动物的活跃期）开始时达到峰值，这表明 CTC 的释放可能受到昼夜节律的调节。
Circulating tumor cells (CTCs) is an established biomarker of cancer metastasis. The circulation dynamics of CTCs are important for understanding the mechanisms underlying tumor cell dissemination. Although studies have revealed that the circadian rhythm may disrupt the growth of tumors, it is generally unclear whether the circadian rhythm controls the release of CTCs. In clinical examinations, the current in vitro methods for detecting CTCs in blood samples are based on a fundamental assumption that CTC counts in the peripheral blood do not change significantly over time, which is being challenged by recent studies. Since it is not practical to draw blood from patients repeatedly, a feasible strategy to investigate the circadian rhythm of CTCs is to monitor them by in vivo detection methods. Fluorescence in vivo flow cytometry (IVFC) is a powerful optical technique that is able to detect fluorescent circulating cells directly in living animals in a noninvasive manner over a long period of time. In this study, we applied fluorescence IVFC to monitor CTCs noninvasively in an orthotopic mouse model of human prostate cancer. We observed that CTCs exhibited stochastic bursts over cancer progression. The probability of the bursting activity was higher at early stages than at late stages. We longitudinally monitored CTCs over a 24-h period, and our results revealed striking daily oscillations in CTC counts that peaked at the onset of the night (active phase for rodents), suggesting that the release of CTCs might be regulated by the circadian rhythm.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
14.8
作者：
通讯作者：
--
淋巴细胞昼夜节律的时钟控制淋巴结运输和适应性免疫反应。
影响因子：
32.4
作者：
Druzd D;Matveeva O;Ince L;Harrison U;He W;Schmal C;Herzel H;Tsang AH;Kawakami N;Leliavski A;Uhl O;Yao L;Sander LE;Chen CS;Kraus K;de Juan A;Hergenhan SM;Ehlers M;Koletzko B;Haas R;Solbach W;Oster H;Scheiermann C
通讯作者：
Scheiermann C
影响因子：
11.2
作者：
Fan, Zhi-Chao;Yan, Jun;Wei, Xun-Bin
通讯作者：
Wei, Xun-Bin
影响因子：
3
作者：
Eden, Uri T.;Kramer, Mark A.
通讯作者：
Kramer, Mark A.