HORMONAL-CONTROL OF BONE COLLAGEN-SYNTHESIS INVITRO - EFFECTS OF INSULIN AND GLUCAGON
HORMONAL-CONTROL OF BONE COLLAGEN-SYNTHESIS INVITRO - EFFECTS OF INSULIN AND GLUCAGON
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DOI：
10.1210/endo-100-3-668
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发表时间：
1977-01-01
期刊：
影响因子：
4.8
通讯作者：
RAISZ, LG
中科院分区：
文献类型：
--
作者：
CANALIS, EM;DIETRICH, JW;RAISZ, LG
研究了猪胰岛素和胰高血糖素对21日龄胎鼠颅骨组织骨胶原蛋白和非胶原蛋白合成的直接影响。骨孵育24-96 h，最后2 h加入[3H]脯氨酸。用纯化的细菌胶原酶测定胶原酶可消化蛋白（CDP）和非胶原蛋白（NCP）中标签的掺入情况。在10-9-10-6 m浓度下，胰岛素使CDP的标记增加了60 - 115%，而对NCP的刺激作用较小。对CDP的影响在培养12-24小时后出现，在激素持续存在的情况下维持96小时，但在从培养基中去除胰岛素后3小时内消失。胰岛素似乎对胶原合成有直接影响，而对胶原分解没有影响。胰岛素不影响[3H]尿苷或[3H]胸苷在24 h时进入骨RNA和DNA片段。胰岛素对甲状旁腺激素和二丁基环AMP的抑制作用有拮抗作用，异丁基甲基黄嘌呤对CDP标记的抑制作用较小。胰高血糖素不影响对胰岛素的反应，其本身对脯氨酸掺入的抑制作用小而可变。
The direct effects of porcine insulin and glucagon on bone collagen and non-collagen protein synthesis were examined in cultures of calvaria obtained from 21 day fetal rats. Bones were incubated for 24-96 h, and [3H]proline was added for the last 2 h of culture. Incorporation of the label into collagenase-digestible protein (CDP) and noncollagen protein (NCP) was determined using purified bacterial collagenase. Insulin increased the labeling of CDP by 60 to 115% at concentrations of 10-9-10-6 M. A smaller stimulatory effect was observed on NCP. The effect on CDP appeared after 12-24 h of culture, was maintained for 96 h in the continuous presence of the hormone, but was lost within 3 h of removal of insulin from the culture medium. Insulin appeared to have a direct effect on collagen synthesis and not on collagen breakdown. Insulin did not affect the incorporation of [3H]uridine or [3H]thymidine into the RNA and DNA fractions of bone at 24 h. Insulin opposed the inhibitory effects of parathyroid hormone and dibutyryl cyclic AMP and to a lesser extent, the inhibitory effect of isobutylmethylxanthine on the labeling of CDP. Glucagon did not affect the response to insulin and by itself had small and variable inhibitory effects on proline incorporation.