Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.
Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.
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通过 DNA 菌落杂交检测产肠毒素大肠杆菌。
DOI：
10.1093/infdis/142.6.892
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Falkow,S
中科院分区：
文献类型：
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作者：
Moseley,SL;Huq,I;Alim,AR;So,M;Samadpour-Motalebi,M;Falkow,S
描述了一种检测大量产肠毒素大肠杆菌分离株的方法，其中检测编码肠毒素的基因，而不是毒素本身。用放射性标记的编码热不稳定（LT）或热稳定（ST）毒素的DNA片段作为杂交探针，在大肠杆菌中进行同源DNA序列的杂交。在硝酸纤维素滤膜上原位生长并裂解的大肠杆菌。LT探针检测到所有31E。ST探针检出17株仅产ST的菌株中的12株，26株同时产ST和LT的菌株中的3株。大肠杆菌同源，人源E.大肠杆菌产生至少两种在幼鼠测定中可检测的异源ST。该杂交方法还检测到肠促炎性E的存在。急性腹泻患者直斑粪便中大肠杆菌的生长。
A method for detecting large numbers of isolates of enterotoxigenicEscherichia coliis described in which the genes encoding the enterotoxins are detected, rather than the toxins themselves. Radiolabeled fragments of DNA encoding the heat-labile (LT) or heatstable (ST) toxins were used as hybridization probes for homologous DNA sequences inE. colicolonies grown and lysed in situ on nitrocellulose filters. The LT probe detected all of 31E. colistrains producing ST and LT or only LT, while the ST probe detected 12 of 17 strains producing only ST and three of 26 strains producing ST and LT. These results suggest that the LTs produced by different isolates ofE. coliare homologous and that human isolates ofE. coliproduce at least two heterologous STs detectable in the infant mouse assay. The hybridization method also detected the presence of enterotoxigenicE. coliin bacterial growth in directly spotted stools from patients with acute diarrhea.