Intranasal administration of nerve growth factor ameliorate β-amyloid deposition after traumatic brain injury in rats
Intranasal administration of nerve growth factor ameliorate β-amyloid deposition after traumatic brain injury in rats
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DOI：
10.1016/j.brainres.2011.12.059
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发表时间：
2012-02-27
期刊：
影响因子：
2.9
通讯作者：
Liu, Xinfeng
中科院分区：
文献类型：
--
作者：
Tian, Lili;Guo, Ruibing;Liu, Xinfeng
创伤性脑损伤(TBI)后淀粉样β蛋白(Aβ)显著升高，是阿尔茨海默病(AD)患者晚年的危险因素。神经生长因子(NGF)在脑损伤修复中具有很大的潜力。但由于缺乏将NGF导入脑内的可行方法，限制了其临床应用。本研究探讨鼻腔注射神经生长因子(NGF)对脑外伤大鼠损伤侧皮质和海马区Aβ负荷的影响。成年雄性SD大鼠采用改良Feeney‘s体重下降模型，鼻腔给予NGF或NGF治疗。分别进行运动和认知功能结果、免疫组织化学染色、酶联免疫吸附试验和免疫印迹检测。与假手术大鼠相比，大鼠脑损伤后APP和Aβ(42)的表达显著增加，功能转归明显降低。鼻腔注射NGF显著减少了Aβ(42)的沉积，并改善了脑外伤后的功能结果。因此，鼻腔注射NGF为降低脑外伤患者晚年发展为AD的风险提供了一种潜在的策略。(C)2012爱思唯尔B.V.保留所有权利。
The marked increase of amyloid-beta (A beta) peptide after traumatic brain injury (TBI), confers a risk factor for Alzheimer's disease (AD) in patients' later life. Nerve growth factor (NGF) is great potential to repair brain injury. But its clinical application is limited because of lacking feasible methods for delivering NGF into brain. This study investigated the effects of NGF, delivered intranasally, on the A beta burden in the injured ipsilateral cortex and hippocampus of rats with TBI. Adult male Sprague-Dawley rats were subjected to the modified Feeney's weight-drop model and treated without or with NGF by intranasal route. Motor and cognitive functional outcome, immunostaining, ELISA assay and western blot were performed. Compared to sham operated rats, TBI rats exhibited significantly increased APP and A beta(42) expression as well as decreased functional outcome after TBI. Intranasal administration of NGF significantly attenuated A beta(42) deposits, and improved functional outcome after TBI. Thus, intranasal delivery of NGF provides a potential strategy for reducing the risk of developing AD in the later life of TBI patients. (C) 2012 Elsevier B.V. All rights reserved.