VASCULAR-PERMEABILITY FACTOR (VASCULAR ENDOTHELIAL GROWTH-FACTOR) EXPRESSION AND ANGIOGENESIS IN CERVICAL NEOPLASIA
VASCULAR-PERMEABILITY FACTOR (VASCULAR ENDOTHELIAL GROWTH-FACTOR) EXPRESSION AND ANGIOGENESIS IN CERVICAL NEOPLASIA
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DOI：
10.1093/jnci/87.16.1237
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发表时间：
1995-08-16
期刊：
影响因子：
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通讯作者：
BROWN, LF
中科院分区：
文献类型：
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作者：
GUIDI, AJ;ABUJAWDEH, G;BROWN, LF
背景：血管生成是实体瘤（包括宫颈癌）进展的关键因素。然而，宫颈肿瘤中血管生成的机制尚未明确。目的：我们的目标是确定宫颈肿瘤中血管生成与血管生成细胞因子血管通透因子（VPF）（也称为血管内皮生长因子）及其受体表达之间的关系。方法：对 66 份宫颈活检标本进行了评估；其中，16个样本被指定为良性，17个样本被指定为低度鳞状上皮内病变，18个样本被指定为高度鳞状上皮内病变，15个样本被指定为浸润性鳞状细胞癌。定量评估因子 VIII 相关抗原免疫染色的组织学切片的微血管密度和上皮基质血管套囊的存在。还通过原位杂交评估切片的 VPF 信使 RNA (mRNA) 表达。结果：与低级别上皮内病变和良性鳞状上皮相比，侵袭性癌和高级上皮内病变中的 VPF mRNA 表达、上皮间质血管套囊和微血管密度计数显着增加。血管套扎和微血管密度计数增加也与 VPF mRNA 表达增加显着相关。结论：这些观察结果表明，VPF 是宫颈肿瘤中重要的血管生成因子。
Background: Angiogenesis is a critical factor in the progression of solid tumors, including cervical cancers. The mechanisms responsible for angiogenesis in cervical neoplasia, however, are not well defined. Purpose: Our goal was to determine the relationship between angiogenesis and the expression of the angiogenic cytokine vascular permeability factor (VPF), also known as vascular endothelial growth factor, and its receptors in cervical neoplasia. Methods: Sixty-six cervical biopsy specimens were evaluated; among these, 16 samples were designated as benign, 17 as low-grade squamous intraepithelial lesions, 18 as high-grade squamous intraepithelial lesions, and 15 as invasive squamous cell carcinomas. Histologic sections immunostained for factor VIII-related antigen were evaluated quantitatively for microvessel density and for the presence of epithelial-stromal vascular cuffing. Sections were also evaluated for VPF messenger RNA (mRNA) expression by in situ hybridization. Results: VPF mRNA expression, epithelial-stromal vascular cuffing, and microvessel density counts were significantly increased in invasive carcinoma and in high-grade intraepithelial lesions as compared with low-grade intraepithelial lesions and benign squamous epithelium. Vascular cuffing and increased microvessel density counts were also significantly associated with increased VPF mRNA expression. Conclusions: These observations suggest that VPF is an important angiogenic factor in cervical neoplasia.