Supplementation with vitamin C and N-acetyl-cysteine increases oxidative stress in humans after an acute muscle injury induced by eccentric exercise
Supplementation with vitamin C and N-acetyl-cysteine increases oxidative stress in humans after an acute muscle injury induced by eccentric exercise
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DOI：
10.1016/s0891-5849(01)00640-2
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发表时间：
2001-09-15
影响因子：
7.4
通讯作者：
Leeuwenburgh, C
中科院分区：
文献类型：
--
作者：
Childs, A;Jacobs, C;Leeuwenburgh, C
目前还没有研究来确定广泛使用的非处方水溶性抗氧化剂维生素C和N-乙酰半胱氨酸（NAC）是否可以在炎症条件下作为人类的促氧化剂。我们通过偏心性手臂肌肉损伤诱导急性期炎症反应。炎症的特征为水肿、肿胀、疼痛和血浆炎症指标、髓过氧化物酶和白细胞介素-6升高。受试者在受伤后立即服用安慰剂或维生素C（12.5 mg/kg体重）和NAC（10 mg/kg体重），持续7天。由此产生的肌肉损伤引起血清博莱霉素可检测铁水平升高，维生素C和NAC组的铁含量较高。乳酸脱氢酶（LDH）、肌酸激酶（CK）浓度。(CK)肌红蛋白在伤后2、3、4 d显著升高，第7天恢复到基线水平。此外，维生素C和NAC组LDH和CK活性升高的程度更大。氧化应激标志物（脂质氢过氧化物和8-异前列腺素F-2 α; 8-Iso-PGF（2 α））和抗氧化酶活性的水平也在损伤后升高。接受维生素C和NAC的受试者在运动后2 d具有较高的脂质过氧化氢和8-Iso-PGF（2 α）水平。这种急性人类炎症模型强烈表明，损伤后立即补充维生素C和NAC会短暂增加组织损伤和氧化应激。(C)2001 Elsevier Science Inc.
There has been no investigation to determine if the widely used over-the-counter, water-soluble antioxidants vitamin C and N-acetyl-cysteine (NAC) could act as pro-oxidants in humans during inflammatory conditions. We induced an acute-phase inflammatory response by an eccentric arm muscle injury. The inflammation was characterized by edema, swelling, pain, and increases in plasma inflammatory indicators, myeloperoxidase and interleukin-6. Immediately following the injury, subjects consumed a placebo or vitamin C (12.5 mg/kg body weight) and NAC (10 mg/kg body weight) for 7 d. The resulting muscle injury caused increased levels of serum bleomycin-detectable iron and the amount of iron was higher in the vitamin C and NAC group. The concentrations of lactate dehydrogenase (LDH), creatine kinase. (CK), and myoglobin were significantly elevated 2, 3, and 4 d postinjury and returned to baseline levels by day 7. In addition, LDH and CK activities were elevated to a greater extent in the vitamin C and NAC group. Levels of markers for oxidative stress (lipid hydroperoxides and 8-iso prostaglandin F-2 alpha; 8-Iso-PGF(2 alpha)) and antioxidant enzyme activities were also elevated post-injury. The subjects receiving vitamin C and NAC had higher levels of lipid hydroperoxides and 8-Iso-PGF(2 alpha) 2 d after the exercise. This acute human inflammatory model strongly suggests that vitamin C and NAC supplementation immediately post-injury, transiently increases tissue damage and oxidative stress. (C) 2001 Elsevier Science Inc.