IDENTIFICATION OF 2 GTP-BINDING PROTEINS IN THE CHLOROPLAST PROTEIN IMPORT MACHINERY
IDENTIFICATION OF 2 GTP-BINDING PROTEINS IN THE CHLOROPLAST PROTEIN IMPORT MACHINERY
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DOI：
10.1126/science.7973656
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发表时间：
1994-11-11
期刊：
影响因子：
56.9
通讯作者：
SCHNELL, DJ
中科院分区：
文献类型：
--
作者：
KESSLER, F;BLOBEL, G;SCHNELL, DJ
在叶绿体外膜转运过程中与转运中间体相关的四种蛋白质中，有两种被鉴定为三磷酸鸟苷（GTP）结合蛋白。这两种蛋白质都是叶绿体外膜的完整膜蛋白，并且都部分暴露在叶绿体表面上，在那里它们可以被嗜热菌蛋白酶消化。外膜的进口机械的进口基板的接合被抑制缓慢水解或非水解GTP类似物。因此，这些GTP结合蛋白可能在蛋白质输入叶绿体中起作用。
Two of four proteins that associated with translocation intermediates during protein import across the outer chloroplast envelope membrane were identified as guanosine triphosphate (GTP)-binding proteins. Both proteins are integral membrane proteins of the outer chloroplast membrane, and both are partially exposed on the chloroplast surface where they were accessible to thermolysin digestion. Engagement of the outer membrane's import machinery by an import substrate was inhibited by slowly hydrolyzable or non-hydrolyzable GTP analogs. Thus, these GTP-binding proteins may function in protein import into chloroplasts.