Complete nucleotide sequence of four RNA segments of fig mosaic virus
Complete nucleotide sequence of four RNA segments of fig mosaic virus
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DOI：
10.1007/s00705-009-0509-3
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发表时间：
2009-11-01
影响因子：
2.7
通讯作者：
Martelli, Giovanni P.
中科院分区：
文献类型：
--
作者：
Elbeaino, Toufic;Digiaro, Michele;Martelli, Giovanni P.
测定了无花果花叶病毒（FMV）4个病毒RNA片段的全序列。四种RNA中的每一种均包含单个开放阅读框（ORF），大小分别为7，093、2，252、1，490和1，472个核苷酸。这些ORF编码的蛋白质依次为：RNA依赖的RNA聚合酶（p1 264 kDa）、一种推定的糖蛋白（p2 73 kDa）、一种推定的核衣壳蛋白（p3 35 kDa）和一种功能未知的蛋白质（p4 40.5 kDa）。所有的RNA片段都具有在50和30末端含有13-nt互补序列的非翻译区。在RNA-1中，除了5个RdRp基序A-F之外，还发现了一个保守的基序，称为前基序A。在用RNA-1和RNA-2的氨基酸序列构建的系统发育树中，FMV与欧洲山灰环斑相关病毒（EMARaV）在一个分支中聚在一起，该分支与汉坦病毒属、正布尼亚病毒属和番茄斑萎病毒属的成员接近。由RNA-3编码的推定的FMV核衣壳蛋白的氨基酸序列与EMARaV和未分类的病毒木豆不育花叶病毒（PPSMV）和玉米红条病毒（MRSV）的可比序列共享同一性。核衣壳序列使这四种病毒在一个接近番茄斑萎病毒属的分支中扎根。根据分子生物学、形态学和流行病学特征，FMV与PPSMV和MRSV的关系非常密切。所有这些病毒在遗传学上都与EMARaV相关，因此似乎有资格被分类在拟议的埃玛病毒属中，而埃玛病毒属又可能在布尼亚病毒科中找到分类学分配。
The complete sequence of four viral RNA segments of fig mosaic virus (FMV) was determined. Each of the four RNAs comprises a single open reading frame (ORF) 7,093, 2,252, 1,490 and 1,472 nucleotides in size, respectively. These ORFs encode the following proteins in the order: RNA-dependent RNA polymerase (p1 264 kDa), a putative glycoprotein (p2 73 kDa), a putative nucleocapsid protein (p3 35 kDa) and a protein with unknown function (p4 40.5 kDa). All RNA segments possess untranslated regions containing at the 50 and 30 termini a 13-nt complementary sequence. A conserved motif denoted premotif A was found to be present in addition to the five RdRp motifs A-F in RNA-1. In phylogenetic trees constructed with the amino acid sequences of RNA-1 and RNA-2, FMV clustered consistently with European mountain ash ringspot-associated virus (EMARaV) in a clade close to those comprising members of the genera Hantavirus, Orthobunyavirus and Tospovirus. The amino acid sequence of the putative FMV nucleocapsid protein encoded by RNA-3 shared identity with comparable sequences of EMARaV and the unclassified viruses pigeonpea sterility mosaic virus (PPSMV) and maize red stripe virus (MRSV). The nucleocapsid sequences rooted the four viruses in a clade close to the genus Tospovirus. Based on molecular, morphological and epidemiological features, FMV appears to be very closely related to PPSMV and MRSV. All these viruses are phylogenetically related to EMARaV and therefore seem to be eligible for classification in the proposed genus Emaravirus, which, in turn, may find a taxonomic allocation in the family Bunyaviridae.