Cervical Expression of Elafin and SLPI in Pregnancy and Their Association With Preterm Labor
Cervical Expression of Elafin and SLPI in Pregnancy and Their Association With Preterm Labor
复制标题
DOI：
10.1111/aji.12354
复制
发表时间：
2015-06-01
影响因子：
3.6
通讯作者：
Fujii, Tomoyuki
中科院分区：
文献类型：
--
作者：
Itaoka, Nao;Nagamatsu, Takeshi;Fujii, Tomoyuki
Elafin和分泌性白细胞肽酶抑制剂（SLPI）是抗菌肽（AMPs）中比较独特的两种。本研究的目的是确定这些AMP的表达水平在宫颈在怀孕期间，并探讨其与早产的关联studyMethod的宫颈上皮细胞拭子从正常孕妇评估的生理表达的弹力素和SLPI。进行横断面分析，以比较三个妇女组，对照组（n=26），妇女与先兆早产谁在长期交付（t-TPL，n=23）和TPL谁在早产结束（p-TPL，n=19）之间的SLPI和弹性蛋白的宫颈表达水平。宫颈SLPI表达水平在妊娠过程中增加，而弹性蛋白酶水平保持不变。子宫颈弹力素和SLPI的mRNA表达水平显着高于在p-TPL相比，t-TPL和control groups. Conclusionconstitutional表达弹力素和SLPI在子宫颈细胞在妊娠期间表明其在局部组织稳态和免疫防御的重要作用。早产妇女宫颈弹性蛋白和SLPI表达的升高可能反映了早产前病原体侵入宫颈的局部反应。
ProblemElafin and secretory leukocyte peptidase inhibitor (SLPI) are unique among antimicrobial peptides (AMPs). This study aimed to determine the expression levels of these AMPs at the cervix during pregnancy and to investigate their association with preterm labor.Method of studyCervical epithelial cells were swabbed from normal pregnant women to evaluate the physiological expression of elafin and SLPI. Cross-sectional analysis was conducted to compare cervical expression levels for SLPI and elafin among three women's groups, controls (n=26), women with threatened preterm labor who delivered at term (t-TPL, n=23) and TPL who ended in preterm labor (p-TPL, n=19).ResultsElafin and SLPI proteins were detected in the squamous and glandular cells of the cervix. Cervical SLPI expression levels increased over the course of pregnancy, whereas elafin levels remained unchanged. Cervical mRNA expression levels of elafin and SLPI were significantly higher in p-TPL compared with t-TPL and control groups.ConclusionConstitutive expression of elafin and SLPI in cervical cells during pregnancy suggests their essential roles in local tissue homeostasis and immune defense. The elevations in cervical elafin and SLPI expression in the women with preterm delivery might reflect the local response to the pathogen invasion into the cervix preceding preterm labor.