Comprehensive Evaluation of the Oral Health Status, Salivary Gland Function, and Oxidative Stress in the Saliva of Patients with Subacute Phase of Stroke: A Case-Control Study
Comprehensive Evaluation of the Oral Health Status, Salivary Gland Function, and Oxidative Stress in the Saliva of Patients with Subacute Phase of Stroke: A Case-Control Study
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脑卒中亚急性期患者口腔健康状况、唾液腺功能及唾液氧化应激综合评价的病例对照研究
DOI：
10.3390/jcm9072252
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发表时间：
2020-07-01
影响因子：
3.9
通讯作者：
Hojan, Katarzyna
中科院分区：
文献类型：
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作者：
Gerreth, Piotr;Maciejczyk, Mateusz;Hojan, Katarzyna
这是第一项全面评估中风患者口腔健康状况、唾液腺功能以及酶和非酶抗氧化防御，以及对非刺激性(NWS)和刺激性(SWS)全唾液中蛋白质和脂质的氧化损伤的研究。这项研究包括30名中风亚急性期患者和一组年龄和性别匹配的对照组。我们发现卒中患者的NWS和SWS患者的抗氧化酶(过氧化氢酶和唾液过氧化物酶)的活性都显著较高，与尿酸浓度相似。然而，在研究组中，SWS中还原型谷胱甘肽(GSH)浓度下降。NWS和SWS患者的糖氧化产物(晚期糖基化终产物(AGE)和蛋白氧化产物(AOPP))和脂质过氧化产物的含量明显高于正常对照组(P<0.05)。在研究组中，刺激唾液分泌和总蛋白含量也有所下降。有趣的是，蛋白质和脂肪氧化产物与SWS流量呈负相关。ROC分析显示，唾液GSH以100%的特异度和100%的敏感度区分分析组(AUC=1.0)。综上所述，在亚急性中风患者中，非刺激性和刺激性唾液中存在氧化还原失衡和蛋白质和脂质的氧化损伤。中风患者还患有唾液腺功能障碍。
This is the first study to assess, comprehensively, the oral health status; salivary glands' function and enzymatic and non-enzymatic antioxidant defense; and oxidative damage to proteins and lipids in the non-stimulated (NWS) and stimulated (SWS) whole saliva of stroke patients. The study included 30 patients in the subacute phase of the stroke and an age and gender-matched control group. We showed that the activity of antioxidant enzymes (catalase and salivary peroxidase) was significantly higher in both NWS and SWS of stroke patients, similarly to uric acid concentration. However, in the study group, the reduced glutathione (GSH) concentration in SWS decreased. The contents of protein glycooxidation products (advanced glycation end products (AGE) and protein oxidation products (AOPP)) and lipid hydroperoxides were significantly higher in NWS and SWS of stroke patients. In the study group there was also a decrease in stimulated saliva secretion and total protein content. Interestingly, products of protein and lipid oxidation correlate negatively with SWS flow. The ROC analysis showed that salivary GSH with 100% specificity and 100% sensitivity differentiates the analyzed groups (AUC = 1.0). To sum up, in subacute stroke patients there are redox imbalances and oxidative damage to proteins and lipids in non-stimulated and stimulated saliva. Stroke patients also suffer from salivary gland dysfunction.