Evaluation of a novel kit for the rapid detection of extended-spectrum beta-lactamases
Evaluation of a novel kit for the rapid detection of extended-spectrum beta-lactamases
复制标题
快速检测广谱 β-内酰胺酶的新型试剂盒的评估
DOI：
10.1007/s10096-005-0074-y
复制
发表时间：
2006
影响因子：
4.5
通讯作者：
R. Kubo
中科院分区：
文献类型：
--
作者：
R. Colodner;B. Reznik;V. Gal;H. Yamazaki;H. Hanaki;R. Kubo
为快速检测重症感染患者革兰阴性杆菌产超广谱β-内酰胺酶（ESBL）的方法，本研究设计了一种新型的商品化检测试剂盒。Cica-Beta Test I（Kanto Chemical，Tokyo，Japan）旨在通过15分钟方案直接从分离的菌落中快速检测革兰氏阴性菌中的ESBL。本研究共检测了304株克雷伯菌属，大肠埃希菌和奇异变形杆菌进行了测试，使用新的试剂盒和表型确证纸片试验，使用头孢噻肟和头孢他啶与和不克拉维辛。与纸片法相比，该试剂盒的敏感性和特异性分别为95.5%和98.1%，因此被证明是一种合适的快速检测ESBL的工具。
Since a method of rapidly detecting extended-spectrum beta-lactamase (ESBL) production in gram-negative isolates from patients with severe infection is urgently required, the present study of a novel commercial kit was conducted. The Cica-Beta Test I (Kanto Chemical, Tokyo, Japan) is designed for the rapid detection of ESBL in gram-negative bacteria directly from isolated colonies in a 15-min protocol. In this study, a total of 304 strains of Klebsiella spp., Escherichia coli and Proteus mirabilis were tested using the novel kit and the phenotypic confirmatory disk test using cefotaxime and ceftazidime with and without clavulanate. The kit showed 95.5 and 98.1% sensitivity and specificity, respectively, as compared to the disk test, and thus proved to be an appropriate tool for the rapid detection of ESBL.