Biological control of an insect pest by gut‐colonizing Enterobacter cloacae transformed with ice nucleation gene
Biological control of an insect pest by gut‐colonizing Enterobacter cloacae transformed with ice nucleation gene
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DOI：
10.1046/j.1365-2672.2000.00904.x
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发表时间：
2000-01
影响因子：
4
通讯作者：
K. Watanabe;K. Abe;M. Sato
中科院分区：
文献类型：
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作者：
K. Watanabe;K. Abe;M. Sato
K.渡边ABE和M. SATO.2000.将附生植物菠萝欧文氏菌（Erwinia（Pantoea）ananas IN 10）的冰核（IN）基因inaA转化到来源于蚕粪便的阴沟肠杆菌WBMH-3-CMr中。命名为阴沟肠杆菌WBMH-3-CMr（pICE 6S 13）的菌株显示IN活性，与亲本菌株不同。将转基因菌株接种于桑螟幼虫体内，用离体桑葚饲养，测定了桑螟幼虫的过冷能力和抗寒性。转基因品系的幼虫平均过冷却点（SCP）为
K. WATANABE, K. ABE and M. SATO.2000. The ice nucleation (IN) gene inaA of epiphytic Erwinia (Pantoea) ananas IN10 was transformed into Enterobacter cloacae WBMH‐3‐CMr originated from the faeces of silkworms. The transformant designated as Ent. cloacae WBMH‐3‐CMr (pICE6S13) exhibited IN activity, unlike the parent strain. The transgenic strain was ingested by mulberry pyralid larvae, fed on detached mulberry leaves, and the supercooling capacity and cold hardiness of these larvae were examined. The mean supercooling point (SCP) of the larvae ingesting the transgenic strain was