Synthesis and characterization of Ciprofloxacin-containing divinyl oligomers and assessment of their biodegradation in simulated salivary esterase
Synthesis and characterization of Ciprofloxacin-containing divinyl oligomers and assessment of their biodegradation in simulated salivary esterase
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DOI：
10.1016/j.dental.2018.01.021
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发表时间：
2018-05-01
期刊：
影响因子：
5
通讯作者：
Santerre, J. Paul
中科院分区：
文献类型：
--
作者：
Delaviz, Yasaman;Nascimento, Mitchell A.;Santerre, J. Paul
目的：导致牙齿修复替换的两个主要原因是复合材料/牙本质界面的边缘破裂和细菌介导的二次龋齿。本研究的目的是合成的低聚物，其中纳入增强的生物稳定性，但也将能够产生抗菌功能，如果他们经历degradation.Methods：稳定性被纳入到低聚物通过产生的结构特征，将物理上阻碍水解敏感基团的可用性的低聚物。作为抗菌特征的概念验证，通过将环丙沙星（CF）共价结合到交联二乙烯基低聚物（称为EDV和HLH-CFPEG）的主链中来实现抗菌功能。在模拟人唾液酯酶中研究了药物寡聚体的水解稳定性，并与商业单体2，2-双[4（2-羟基-3-甲基丙烯酰氧基丙氧基）苯基]丙烷（BisGMA）进行了比较。降解后，两种药物低聚物以游离形式差异释放CF。从树脂配方中含有15重量%的HLH-CFPEG的聚合物合成表现出高度的乙烯基转化率和凝胶含量，并在水解条件下显示释放CF在28天的监测studyperiod.Significance：HLH-CFPEG可用于牙科树脂粘合剂系统的局部交付CF的边缘接口。最大限度地减少变形链球菌在边缘部位的生长，可以通过降低特异性来源于链球菌的酯酶活性来延长寿命。变异人(C)2018年牙科材料学院。爱思唯尔公司出版All rights reserved.
Objective: Two leading causes contributing to dental restoration replacement are the marginal breakdown at the composite/dentin interface and secondary caries mediated by bacteria. The objective of the present study was to synthesize oligomers which incorporated enhanced bio-stability but would also be able to generate antimicrobial function if they underwent degradation.Methods: Stability was incorporated into the oligomers by generating structural features that would physically hinder the availability of hydrolytically sensitive groups in the oligomers. As a proof-of concept for the antibacterial feature, antimicrobial function was achieved by covalently incorporating Ciprofloxacin (CF) into the backbone of cross-linking divinyl oligomers (referred to as EDV and HLH-CFPEG). The hydrolytic stability of the oligomers was studied in simulated human salivary esterase and compared to the commercial monomer 2,2-bis[4(2-hydroxy-3-methacryloxypropoxy)-phenyl]propane (BisGMA).Results: Both drug oligomers were found to be significantly more stable than BisGMA. Upon degradation, both drug oligomers released CF differentially in free form. Polymer synthesis from resin formulations containing 15 wt% HLH-CFPEG showed a high degree of vinyl group conversion and gel content, and under hydrolytic conditions showed the release of CF during a 28-day monitoring study period.Significance: HLH-CFPEG can be used in dental resin adhesive systems for local delivery of CF to the marginal interface. Minimizing the growth of Streptococcus mutans at the marginal site can improve longevity by reducing esterase activity derived specifically from S. mutans. (C) 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.