A quantitative method for analyzing establishing‐efficiency of persistent viral infection
A quantitative method for analyzing establishing‐efficiency of persistent viral infection
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一种定量分析持续性病毒感染建立效率的方法
DOI：
10.1111/j.1348-0421.2009.00122.x
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发表时间：
2009
影响因子：
2.6
通讯作者：
Yasuhiko Ito
中科院分区：
文献类型：
--
作者：
Morihiro Ito;Izumi Yamakawa;M. Nishio;M. Tsurudome;M. Kawano;H. Komada;Yasuhiko Ito
设计了一种定量分析持续感染建立效率的方法。发现hPIV2 CA和SV5 T1毒株的效率较高，即0.1 ≤ 0.3（效率1.0表明100%的病毒感染细胞持续感染）。SV5 WR毒株的效率也很高，约为0.1，尽管病毒没有能力在全细胞培养系统中立即建立稳定的持续感染状态。在约0.0007处，SV 41的效率几乎与hPIV 2 Toshiba株的效率相同。进一步详细分析了各种rSeV的建立效率。rSeV（PA）、rSeV（Ppi）和rSeV（HNpi）的效率低于检测限，而rSeV（Lpi）的效率接近1。虽然效率约为0.001，但出乎意料地发现rSeV（Mpi）和rSeV（Fpi）能够形成持续感染的细胞，表明Fpi和Mpi蛋白都有助于SeVpi的持续感染的建立效率。
A quantitative method for analyzing establishing‐efficiency of persistent infection was devised. The efficiency of hPIV2 CA and SV5 T1 strains was found to be high, that is, 0.1∼0.3 (an efficiency of 1.0 indicates that 100% of the virus‐infected cells became persistently infected). The efficiency of the SV5 WR strain was also high, approximately 0.1, though the virus had no ability to immediately establish a steady state of persistent infection in whole cell‐culture systems. At about 0.0007, the efficiency of SV41 was almost the same as that of the hPIV2 Toshiba strain. The establishing efficiencies of various rSeV were further analyzed in detail. The efficiencies of the rSeV(PA), rSeV(Ppi) and rSeV(HNpi) were below the limit of detection, while that of rSeV(Lpi) was nearly 1. Although the efficiency was around 0.001, the rSeV(Mpi) and the rSeV(Fpi) were unexpectedly found to be capable of forming persistently‐infected cells, indicating that both the Fpi and Mpi proteins contribute to the establishing efficiency of persistent infection of SeVpi.